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Production efficiency and properties of bacterial cellulose membranes in a novel grape pomace hydrolysate by Komagataeibacter melomenusus ▫$AV436^T$▫ and Komagataeibacter xylinus LMG 1518Selestina Gorgieva,
Urška Jančič,
Eva Cepec,
Janja Trček, 2023, izvirni znanstveni članek
Opis: The microbial production of cellulose using different bacterial species has been extensively examined for various
industrial applications. However, the cost-effectiveness of all these biotechnological processes is strongly related to the culture medium for bacterial cellulose (BC) production. Herein, we examined a simple and modified
procedure for preparing grape pomace (GP) hydrolysate, without enzymatic treatment, as a sole growth medium
for BC production by acetic acid bacteria (AAB). The central composite design (CCD) was used to optimise the GP
hydrolysate preparation toward the highest reducing sugar contents (10.4 g/L) and minimal phenolic contents
(4.8 g/L). The experimental screening of 4 differently prepared hydrolysates and 20 AAB strains identified the
recently described species Komagataeibacter melomenusus AV436T as the most efficient BC producer (up to 1.24 g/
L dry BC membrane), followed by Komagataeibacter xylinus LMG 1518 (up to 0.98 g/L dry BC membrane). The
membranes were synthesized in only 4 days of bacteria culturing, 1 st day with shaking, followed by 3 days of
static incubation. The produced BC membranes in GP-hydrolysates showed, in comparison to the membranes
made in a complex RAE medium 34 % reduction of crystallinity index with the presence of diverse cellulose
allomorphs, presence of GP-related components within the BC network responsible for the increase of hydrophobicity, the reduction of thermal stability and 48.75 %, 13.6 % and 43 % lower tensile strength, tensile
modulus, and elongation, respectively. Here presented study is the first report on utilising a GP-hydrolysate
without enzymatic treatment as a sole culture medium for efficient BC production by AAB, with recently
described species Komagataeibacter melomenusus AV436T as the most efficient producer in this type of food-waste
material. The scale-up protocol of the scheme presented here will be needed for the cost-optimisation of BC
production at the industrial levels.
Ključne besede: grape pomace hydrolysate, bacterial cellulose, acetic acid bacteria, Komagataeibacter melomenusus
Objavljeno v DKUM: 26.07.2024; Ogledov: 98; Prenosov: 7
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