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1.
PRIPRAVA PODATKOVNE BAZE PROBIOTIČNIH IN PROTEKTURNIH KULTUR
Vanja Žiher, 2012, magistrsko delo

Opis: Probiotične bakterije se med seboj razlikujejo. Ene so uspešnejše pri borbi proti škodljivim bakterijam, druge pri spodbujanju delovanja prebavil, tretje zopet pri spodbujanju imunskega sistema. Vse boljše poznavanje mehanizmov njihovega delovanja in vse več kliničnih raziskav, opravljenih v zadnjih letih, nakazuje možnost uspešne uporabe probiotičnih bakterij tako v preventivi, kot tudi podpori pri zdravljenju različnih bolezni. V magistrski nalogi, smo uporabili anketni vprašalnik in na podlagi pridobljenih rezultatov, smo opisovali lastnosti in klinično uporabo določenih probiotičnih sevov. Zbrani podatki, bodo predstavljeni na spletni strani Fakultete za Kmetijstvo in biosistemske vede Maribor.
Ključne besede: probiotiki, imunski sistem, Lactobacillus, Bifidobacterium, Enterococcus
Objavljeno: 25.05.2012; Ogledov: 1250; Prenosov: 179
.pdf Polno besedilo (1,97 MB)

2.
Microorganisms with claimed probiotic properties: an overview of recent literature
Sabina Fijan, 2014, pregledni znanstveni članek

Opis: Probiotics are defined as live microorganisms, which when administered in adequate amounts, confer a health benefit on the host. Health benefits have mainly been demonstrated for specific probiotic strains of the following genera: Lactobacillus, Bifidobacterium, Saccharomyces, Enterococcus, Streptococcus, Pediococcus, Leuconostoc, Bacillus, Escherichia coli. The human microbiota is getting a lot of attention today and research has already demonstrated that alteration of this microbiota may have far-reaching consequences. One of the possible routes for correcting dysbiosis is by consuming probiotics. The credibility of specific health claims of probiotics and their safety must be established through science-based clinical studies. This overview summarizes the most commonly used probiotic microorganisms and their demonstrated health claims. As probiotic properties have been shown to be strain specific, accurate identification of particular strains is also very important. On the other hand, it is also demonstrated that the use of various probiotics for immunocompromised patients or patients with a leaky gut has also yielded infections, sepsis, fungemia, bacteraemia. Although the vast majority of probiotics that are used today are generally regarded as safe and beneficial for healthy individuals, caution in selecting and monitoring of probiotics for patients is needed and complete consideration of risk-benefit ratio before prescribing is recommended.
Ključne besede: Lactic-acid bacteria, Lactobacillus, Bifidobacterium, Saccharomyces, Enterococcus, Streptococcus, Pediococcus, Leuconostoc, Bacillus, Escherichia coli
Objavljeno: 21.06.2017; Ogledov: 40; Prenosov: 0
.pdf Polno besedilo (267,98 KB)

3.
Effect of Bifidobacterium breve on the intestinal microbiota of coeliac children on gluten free diet
Andrea Quagliariello, Irene Aloisio, Nicole Bozzi Cionci, Donata Luiselli, Giuseppe D'Auria, Llúcia Martinez-Priego, David Pérez-Villarroya, Tomaž Langerholc, Maša Primec, Dušanka Mičetić-Turk, Diana Di Gioia, 2016, izvirni znanstveni članek

Opis: Coeliac disease (CD) is associated with alterations of the intestinal microbiota. Although several Bifidobacterium strains showed anti-inflammatory activity and prevention of toxic gliadin peptides generation in vitro, few data are available on their efficacy when administered to CD subjects. This study evaluated the effect of administration for three months of a food supplement based on two Bifidobacterium breve strains (B632 and BR03) to restore the gut microbial balance in coeliac children on a gluten free diet (GFD). Microbial DNA was extracted from faeces of 40 coeliac children before and after probiotic or placebo administration and 16 healthy children (Control group). Sequencing of the amplified V3-V4 hypervariable region of 16S rRNA gene as well as qPCR of Bidobacterium spp., Lactobacillus spp., Bacteroides fragilis group Clostridium sensu stricto and enterobacteria were performed. The comparison between CD subjects and Control group revealed an alteration in the intestinal microbial composition of coeliacs mainly characterized by a reduction of the Firmicutes/Bacteroidetes ratio, of Actinobacteria and Euryarchaeota. Regarding the effects of the probiotic, an increase of Actinobacteria was found as well as a re-establishment of the physiological Firmicutes/Bacteroidetes ratio. Therefore, a three-month administration of B. breve strains helps in restoring the healthy percentage of main microbial components.
Ključne besede: coeliac disease, gluten free diet, probiotic, Bifidobacterium breve, intestinal microbiota, qPCR, next generation sequencing
Objavljeno: 22.06.2017; Ogledov: 44; Prenosov: 0
.pdf Polno besedilo (5,19 MB)

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