1. Morphological, mechanical, and in-vitro bioactivity of gelatine/collagen/hydroxyapatite based scaffolds prepared by unidirectional freeze-castingYasir Beeran Potta Thara, Tomaž Vuherer, Uroš Maver, Vanja Kokol, 2021, izvirni znanstveni članek Opis: The fabrication of biomaterials to be used in segmental bone defects, mimicking the bone's organic-inorganic architecture and mechanical properties to induce osteogenesis, persists as a key challenge. The purpose of this study was to elucidate the effect of a lightweight, morphologically graded, and multiphase self-standing scaffold structure prepared from a combination of gelatine (Gel), collagen type 1 (Col) and/or hydroxyapatite (HAP) nanoparticles by a unidirectional freeze-casting process at different temperatures (−20, −40, −60 °C), followed by carbodiimide induced cross-linking, on their in-vitro mechanical stability and bioactive properties. In addition, the rheological study of differently formulated Gel solutions has been performed to determine the effect of Col and HAP content on their microstructural arrangement, which, together with the freezing kinetic, affects Gel/Col orientation and cross-linking, and, thus, the scaffold's mechanical strength and stability. A bone-like anisotropic, interconnected, and graded porosity (from 120 to a few μm) scaffold structure with up to 30% total porosity and ~61 μm average pores' diameter is obtained by using a higher Col content (Col: Gel = 2:5) and freezing temperature (−20 °C) while forming a few μm thick close-to-parallel lamellae, separated with a 10–100 μm space when prepared at −60 °C. Such a structure influenced in-vitro stability strongly (lower swelling without weight loss), being accompanied with a ~76% increase of compression strength (to 37 kPa) and ~67% decrease of elastic modulus (to 17 kPa) when prepared with HAP and incubated in HBSS for 7 days. On the other hand, a significant reduction of both strength (~78%, to 15 kPa) and elasticity (~95%, to 5 kPa) was noted for a scaffold prepared with HAP at −60 °C, being related to faster degradation and the formation of a highly opened structure on the bottom, required to stimulate the bone ingrowth, while a more closed network structure on the top to adhere with the surrounding soft tissue. None of the scaffolds induced cytotoxicity to human bone-derived osteoblasts, even after 19 days of incubation, but rather improved their viability while promoting cells' adhesions, proliferation, and differentiation, being supported with an increased alkaline phosphatase activity and rod-like CaP formation.
Ključne besede: biomimetic scaffolds, rheology, unidirectional freeze-casting, morphology, compression properties, bioactivity
Objavljeno v DKUM: 10.03.2025; Ogledov: 0; Prenosov: 3
 Celotno besedilo (22,97 MB)
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2. In vitro disease models of the endocrine pancreasMarko Milojević, Jan Rožanc, Jernej Vajda, Laura Činč Ćurić, Eva Paradiž, Andraž Stožer, Uroš Maver, Boštjan Vihar, 2021, pregledni znanstveni članek Opis: The ethical constraints and shortcomings of animal models, combined with the demand to study disease pathogenesis under controlled conditions, are giving rise to a new field at the interface of tissue engineering and pathophysiology, which focuses on the development of in vitro models of disease. In vitro models are defined as synthetic experimental systems that contain living human cells and mimic tissue- and organ-level physiology in vitro by taking advantage of recent advances in tissue engineering and microfabrication. This review provides an overview of in vitro models and focuses specifically on in vitro disease models of the endocrine pancreas and diabetes. First, we briefly review the anatomy, physiology, and pathophysiology of the human pancreas, with an emphasis on islets of Langerhans and beta cell dysfunction. We then discuss different types of in vitro models and fundamental elements that should be considered when developing an in vitro disease model. Finally, we review the current state and breakthroughs in the field of pancreatic in vitro models and conclude with some challenges that need to be addressed in the future development of in vitro models.
Ključne besede: in vitro disease models, pancreas, islet of Langerhans, 3D cell culture, scaffolds, acute tissue slices
Objavljeno v DKUM: 01.10.2024; Ogledov: 0; Prenosov: 400
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3. Magnetic nanoparticles in 3D-printed scaffolds for biomedical applicationsNina Marovič, Irena Ban, Uroš Maver, Tina Maver, 2023, pregledni znanstveni članek Ključne besede: magnetic nanoparticles, 3D-printed scaffolds, iron oxide nanoparticles, mechanical properties, cellular responses
Objavljeno v DKUM: 20.05.2024; Ogledov: 195; Prenosov: 13
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4. 3D-printed collagen–nanocellulose hybrid bioscaffolds with tailored properties for tissue engineering applicationsAndreja Dobaj-Štiglic, Florian Lackner, Chandran Nagaraj, Marco Beaumont, Matej Bračič, Isabel Duarte, Veno Kononenko, Damjana Drobne, Balaraman Madhan, Matjaž Finšgar, Rupert Kargl, Karin Stana-Kleinschek, Tamilselvan Mohan, 2023, izvirni znanstveni članek Ključne besede: assays, degradation, porosity, scaffolds, 3D printing
Objavljeno v DKUM: 09.04.2024; Ogledov: 238; Prenosov: 800
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5. Composite PLGA–nanobioceramic coating on moxifloxacin-loaded akermanite 3D porous scaffolds for bone tissue regenerationGeorgia K. Pouroutzidou, Lambrini Papadopoulou, Maria Lazaridou, Konstantinos Tsachouridis, Chrysanthi Papoulia, Dimitra Patsiaoura, Ioannis Tsamesidis, Konstantinos Chrissafis, George Vourlias, Konstantinos M. Paraskevopoulos, Antonios D. Anastasiou, Dimitrios Bikiaris, Eleana Kontonasaki, 2023, izvirni znanstveni članek Ključne besede: 3D porous scaffolds, foam replica technique, akermanite scaffolds, nanofillers, moxifloxacin- loaded scaffolds
Objavljeno v DKUM: 05.04.2024; Ogledov: 209; Prenosov: 17
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