Iskanje po katalogu digitalne knjižnice

Opis: In this study, a novel biodegradable poly(lactic-co-glycolic) acid coating containing a model anti-inflammatory drug (ketoprofen) was prepared on TiAl6V4 substrate for use in orthopedic medicine by a two-step process combining drop casting and supercritical CO2-assisted foaming. The prepared coating was first investigated by surface analysis techniques using time-of-flight secondary ion mass spectrometry and X-ray photoelectron spectroscopy. The combined results confirmed the loading of ketoprofen and its homogeneous spatial distribution in the coating. 3D profilometry revealed increased surface roughness of the coating compared to the bare TiAl6V4 substrate, which is favorable for cell adhesion. Furthermore, the electrochemical measurements (i.e., electrochemical impedance spectroscopy and potentiodynamic curve measurements) demonstrated that the coating application significantly mitigated corrosion compared to the bare TiAl6V4 substrate. In vitro drug release tests revealed extended drug release in simulated body fluids with zero-order release kinetics. Finally, the promising cell testing results using adipose-derived mesenchymal stem cells and osteoblasts confirmed the applicability of the coating for implants. Overall, the results of this study highlight the significant potential of the developed bioactive coating for future orthopedic applications.
Ključne besede: TiAlV, bioactive coating, implants, PLGA, supercritical foaming, ketoprofen
Objavljeno v DKUM: 14.03.2025; Ogledov: 0; Prenosov: 1
Celotno besedilo (4,80 MB)

Opis: The fabrication of biomaterials to be used in segmental bone defects, mimicking the bone's organic-inorganic architecture and mechanical properties to induce osteogenesis, persists as a key challenge. The purpose of this study was to elucidate the effect of a lightweight, morphologically graded, and multiphase self-standing scaffold structure prepared from a combination of gelatine (Gel), collagen type 1 (Col) and/or hydroxyapatite (HAP) nanoparticles by a unidirectional freeze-casting process at different temperatures (−20, −40, −60 °C), followed by carbodiimide induced cross-linking, on their in-vitro mechanical stability and bioactive properties. In addition, the rheological study of differently formulated Gel solutions has been performed to determine the effect of Col and HAP content on their microstructural arrangement, which, together with the freezing kinetic, affects Gel/Col orientation and cross-linking, and, thus, the scaffold's mechanical strength and stability. A bone-like anisotropic, interconnected, and graded porosity (from 120 to a few μm) scaffold structure with up to 30% total porosity and ~61 μm average pores' diameter is obtained by using a higher Col content (Col: Gel = 2:5) and freezing temperature (−20 °C) while forming a few μm thick close-to-parallel lamellae, separated with a 10–100 μm space when prepared at −60 °C. Such a structure influenced in-vitro stability strongly (lower swelling without weight loss), being accompanied with a ~76% increase of compression strength (to 37 kPa) and ~67% decrease of elastic modulus (to 17 kPa) when prepared with HAP and incubated in HBSS for 7 days. On the other hand, a significant reduction of both strength (~78%, to 15 kPa) and elasticity (~95%, to 5 kPa) was noted for a scaffold prepared with HAP at −60 °C, being related to faster degradation and the formation of a highly opened structure on the bottom, required to stimulate the bone ingrowth, while a more closed network structure on the top to adhere with the surrounding soft tissue. None of the scaffolds induced cytotoxicity to human bone-derived osteoblasts, even after 19 days of incubation, but rather improved their viability while promoting cells' adhesions, proliferation, and differentiation, being supported with an increased alkaline phosphatase activity and rod-like CaP formation.
Ključne besede: biomimetic scaffolds, rheology, unidirectional freeze-casting, morphology, compression properties, bioactivity
Objavljeno v DKUM: 10.03.2025; Ogledov: 0; Prenosov: 2
Celotno besedilo (22,97 MB)
Gradivo ima več datotek! Več...

Opis: V zadnjem času je bil dosežen pomemben napredek pri razvoju ADME (absorpcija, distribucija, metabolizem, ekskrecija) modelov, vendar izziv ostaja vzpostaviti platforme, ki bi zmanjšale testiranje na živalih in stroške raziskav. Naraščajoča pomembnost farmakokinetičnih interakcij poudarja potrebo po zanesljivih in ponovljivih ADME modelih, ki so vse bolj ključni za razvoj zdravil in zagotavljanje varnosti z željo po preprečevanju resnih kliničnih zapletov in hospitalizacije.
Ključne besede: ADME, drug interaction studies, in vitro models, pharmacokinetics, multi-organ models
Objavljeno v DKUM: 07.01.2025; Ogledov: 0; Prenosov: 20
Celotno besedilo (405,16 KB)
Gradivo ima več datotek! Več...
Gradivo je zbirka in zajema 1 gradivo!

Opis: The field of ophthalmology is expanding exponentially, both in terms of diagnostic and therapeutic capabilities, as well as the worldwide increasing incidence of eye-related diseases. Due to an ageing population and climate change, the number of ophthalmic patients will continue to increase, overwhelming healthcare systems and likely leading to under-treatment of chronic eye diseases. Since drops are the mainstay of therapy, clinicians have long emphasised the unmet need for ocular drug delivery. Alternative methods, i.e., with better compliance, stability and longevity of drug delivery, would be preferred. Several approaches and materials are being studied and used to overcome these drawbacks. We believe that drug-loaded contact lenses are among the most promising and are a real step toward dropless ocular therapy, potentially leading to a transformation in clinical ophthalmic practice. In this review, we outline the current role of contact lenses in ocular drug delivery, focusing on materials, drug binding and preparation, concluding with a look at future developments.
Ključne besede: contact lens materials, advanced ocular drug delivery, dropless ocular therapy, drug-laden contact lens
Objavljeno v DKUM: 03.12.2024; Ogledov: 0; Prenosov: 1
Celotno besedilo (4,80 MB)
Gradivo ima več datotek! Več...

Opis: Tissue engineering and regenerative medicine have come a long way in recent decades, but the lack of functioning vasculature is still a major obstacle preventing the development of thicker, physiologically relevant tissue constructs. A large part of this obstacle lies in the development of the vessels on a microscale%the microvasculature%that are crucial for oxygen and nutrient delivery. In this review, we present the state of the art in the field of microvascular tissue engineering and demonstrate the challenges for future research in various sections of the field. Finally, we illustrate the potential strategies for addressing some of those challenges.
Ključne besede: regenerative medicine, tissue engineering, vascularization, biomaterials, microvascularization, coculture, gradients
Objavljeno v DKUM: 18.10.2024; Ogledov: 0; Prenosov: 2
Celotno besedilo (6,81 MB)
Gradivo ima več datotek! Več...