1. Macromolecular inks of nanocellulose-alginate for direct-ink-writing : functional scaffolds enriched with curcumin extractsGal Slaček, Tamilselvan Mohan, Petra Kotnik, Tobias Alexander Steindorfer, Marco Beaumont, Željko Knez, Maša Knez Marevci, Karin Stana-Kleinschek, 2026, izvirni znanstveni članek Opis: We present a scalable platform for the fabrication of multifunctional bioactive materials by integrating, polysaccharide-based matrices, green extraction and additive manufacturing. In this work, bioactive 3D-printed structures were fabricated using inks composed of nanofibrillated cellulose (NFC), alginate (Alg), and curcumin extracts. Bioactive compounds were efficiently recovered from turmeric via solvent-free supercritical carbon dioxide extraction, yielding up to 6.57 mg/100 g curcuminoids and 232.45 mg/100 g total phenols. The extracts exhibited robust antioxidant activity (DPPH• IC50: 23.24 mg/mL; ABTS+• IC50: 3.59 mg/mL) and broad-spectrum antimicrobial efficacy against Staphylococcus aureus, Escherichia coli, Candida albicans, and Pseudomonas aeruginosa (MIC: 9.38 mg/mL). Incorporation into NFC-Alg inks exhibited excellent shear-thinning properties suitable for direct-ink-writing 3D printing. Post-printing CaCl2 crosslinking further reinforced the hydrogel network, enhancing mechanical robustness and shape fidelity. The resulting constructs featured a highly porous, grid-like architecture with intricate surface morphology, highlighting significant potential for biomedical and tissue engineering applications.
Ključne besede: nanocellulose, alginate, curcumin extract, supercritical-CO2, antioxidant, antimicrobial properties, 3D-printing
Objavljeno v DKUM: 08.12.2025; Ogledov: 0; Prenosov: 3
 Celotno besedilo (7,77 MB) |
2. Chemo-enzymatic synthesis of viscoelastic pseudopeptidoglycan networksPhilipp Loibner, David Bučak-Gasser, Katharina Schober, Tobias Alexander Steindorfer, Monika Brandtner, Tobias Dorn, Tanja Wrodnigg, Dmytro Neshchadin, Georg Gescheidt-Demner, Matej Bračič, Florian Lackner, Tamilselvan Mohan, Karin Stana-Kleinschek, Rupert Kargl, 2025, izvirni znanstveni članek Opis: Bacterial peptidoglycans (PGs) are essential targets for antibiotics and immune cells. Chemical methods to reproduce PGs semisynthetically are tedious and wasteful. In this work, we describe a new approach to form pseudo-PGs (PPGs) using the protease papain and custom-made peptides conjugated to a glycan. The kinetics of formation is monitored by rheology and 1H NMR. Viscoelastic gels of controlled strength are formed, depending on the temperature and the number of peptide bridges between the glycan chains. We propose that the new method has a high impact on biomaterials research, since it could be used to deliver peptides, test antibiotic efficacy, or investigate human immune cell response.
Ključne besede: biomaterials, bacterial peptidoglycans (PGs), chemo-enzymatic synthesis
Objavljeno v DKUM: 03.11.2025; Ogledov: 0; Prenosov: 9
Celotno besedilo (4,32 MB)
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3. Antithrombogenic polysaccharide coatings to improve hemocompatibility, protein-repellence, and endothelial cell responseMatej Bračič, Bence M. Nagy, Olivija Plohl, Florian Lackner, Tobias Alexander Steindorfer, Roland Fischer, Thomas Heinze, Andrea Olschewski, Karin Stana-Kleinschek, Chandran Nagaraj, Tamilselvan Mohan, 2024, izvirni znanstveni članek Opis: Polyester biomaterials play a crucial in vascular surgery, but suffer from unspecific protein adsorption,
thrombogenicity, and inadequate endothelial cell response, which limit their success. To address these issues, we investigated the functionalization of polyester biomaterials with antithrombogenic polysaccharide coatings. A two-step and water-based method was used to coat cationized polycaprolactone with
different sulfated polysaccharides (SPS), which resulted in long-term stability, tunable morphology,
roughness, film thickness, chemical compositions, zeta potential, and water content. The coatings significantly increased the anticoagulant activity and reduced the thrombogenicity of polycaprolactone, particularly with highly sulfated heparin and cellulose sulfate. Less SPS, such as chondroitin sulfate, fucoidan,
and carrageenan, despite showing reduced anticoagulant activity, also exhibited lower fibrinogen adsorption. The adhesion and viability of human primary endothelial cells cultured on modified polycaprolactone
correlated with the type and sulfate content of the coatings.
Ključne besede: polyester biomaterials, vascular surgery, antithrombogenic polysaccharide coatings
Objavljeno v DKUM: 10.09.2024; Ogledov: 65; Prenosov: 24
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4. Covalent modification of chitosan surfaces with a sugar amino acid and lysine analoguesTobias Dorn, Matjaž Finšgar, Karin Stana-Kleinschek, Tobias Alexander Steindorfer, Martin Simon Thonhofer, Tanja Wrodnigg, Rupert Kargl, 2024, izvirni znanstveni članek Opis: This work explores the modifcation and characterization of chitosan thin flms as a model for functionalized polysaccharide interfaces. The solid–liquid interface of oligo- and polysaccharides is crucial for various biological processes such as cell adhesion and recognition. By covalent surface modifcation of the chitosan via amide formation with diferent small molecules containing carboxylic acids, e.g. specially designed glycoside hydrolase inhibitors, interactions with biomolecules and living cells could potentially be controlled in the future. As a frst step towards this aim, three fuorescent compounds were conjugated onto nanometric chitosan thin flms. The layers were analysed by fuorescence spectroscopy, X-ray photoelectron spectroscopy, time-of-fight secondary ion mass spectrometry, and atomic force microscopy, to proof the covalent attachment of the target molecules. By this analysis, a uniform and chemically stable covalent attachment of the target molecules on the chitosan thin flms could be demonstrated under various conditions. This publication serves as a proof-of-concept-study for further biofunctionalization, pattering, and interaction studies involving polysaccharide interfaces, glycosidase inhibitors, proteins, or living cells.
Ključne besede: carbohydrates, fluorescence spectroscopy, amino acid, chitosan, thin flm modifcation, C-Glycosides
Objavljeno v DKUM: 07.08.2024; Ogledov: 127; Prenosov: 26
Celotno besedilo (2,03 MB)
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