1. Antithrombogenic polysaccharide coatings to improve hemocompatibility, protein-repellence, and endothelial cell responseMatej Bračič, Bence M. Nagy, Olivija Plohl, Florian Lackner, Tobias Alexander Steindorfer, Roland Fischer, Thomas Heinze, Andrea Olschewski, Karin Stana-Kleinschek, Chandran Nagaraj, Tamilselvan Mohan, 2024, izvirni znanstveni članek Opis: Polyester biomaterials play a crucial in vascular surgery, but suffer from unspecific protein adsorption,
thrombogenicity, and inadequate endothelial cell response, which limit their success. To address these issues, we investigated the functionalization of polyester biomaterials with antithrombogenic polysaccharide coatings. A two-step and water-based method was used to coat cationized polycaprolactone with
different sulfated polysaccharides (SPS), which resulted in long-term stability, tunable morphology,
roughness, film thickness, chemical compositions, zeta potential, and water content. The coatings significantly increased the anticoagulant activity and reduced the thrombogenicity of polycaprolactone, particularly with highly sulfated heparin and cellulose sulfate. Less SPS, such as chondroitin sulfate, fucoidan,
and carrageenan, despite showing reduced anticoagulant activity, also exhibited lower fibrinogen adsorption. The adhesion and viability of human primary endothelial cells cultured on modified polycaprolactone
correlated with the type and sulfate content of the coatings.
Ključne besede: polyester biomaterials, vascular surgery, antithrombogenic polysaccharide coatings
Objavljeno v DKUM: 10.09.2024; Ogledov: 65; Prenosov: 15
 Celotno besedilo (5,07 MB)
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2. Covalent modification of chitosan surfaces with a sugar amino acid and lysine analoguesTobias Dorn, Matjaž Finšgar, Karin Stana-Kleinschek, Tobias Alexander Steindorfer, Martin Simon Thonhofer, Tanja Wrodnigg, Rupert Kargl, 2024, izvirni znanstveni članek Opis: This work explores the modifcation and characterization of chitosan thin flms as a model for functionalized polysaccharide
interfaces. The solid–liquid interface of oligo- and polysaccharides is crucial for various biological processes such as cell
adhesion and recognition. By covalent surface modifcation of the chitosan via amide formation with diferent small molecules containing carboxylic acids, e.g. specially designed glycoside hydrolase inhibitors, interactions with biomolecules and
living cells could potentially be controlled in the future. As a frst step towards this aim, three fuorescent compounds were
conjugated onto nanometric chitosan thin flms. The layers were analysed by fuorescence spectroscopy, X-ray photoelectron
spectroscopy, time-of-fight secondary ion mass spectrometry, and atomic force microscopy, to proof the covalent attachment
of the target molecules. By this analysis, a uniform and chemically stable covalent attachment of the target molecules on
the chitosan thin flms could be demonstrated under various conditions. This publication serves as a proof-of-concept-study
for further biofunctionalization, pattering, and interaction studies involving polysaccharide interfaces, glycosidase inhibitors, proteins, or living cells.
Ključne besede: carbohydrates, fluorescence spectroscopy, amino acid, chitosan, thin flm modifcation, C-Glycosides
Objavljeno v DKUM: 07.08.2024; Ogledov: 127; Prenosov: 20
Celotno besedilo (2,03 MB) |
