Iskanje po katalogu digitalne knjižnice

Opis: Tridimenzionalni (3D) biotiskani modeli kože se vse bolj uveljavljajo v predkliničnih in kliničnih raziskavah kot alternativa testiranju na živalih. Ključno za uspeh teh raziskav je zagotavljanje ponovljivosti in primerljivosti rezultatov, kar zahteva razvoj zanesljivih hidrogelov, ki omogočajo stabilno vključitev celic v 3D biotisk. Eden največjih izzivov pri tem je zagotavljanje konsistentnosti lastnosti hidrogela, ki je temeljni material za izdelavo 3D tiskanega in vitro modela kože, potrebnega za različne faze raziskav. Namen te raziskave je bil preučiti možnost priprave večje serije hidrogela ter raziskati načine njihovega dolgoročnega shranjevanja, s poudarkom na tehnikah zamrzovanja, z namenom ohranitve njihovih fizikalno-kemijskih lastnosti. Tako bi omogočili uporabo enotne serije hidrogela skozi celoten razvoj in vitro modela kože, kar bi zmanjšalo variacije, ki nastajajo pri posameznih pripravah hidrogelov za različne raziskovalne metode. Osredotočili smo se na vpliv temperature (-20 °C in -80 °C) ter trajanja shranjevanja (7 in 29 dni) na ključne lastnosti hidrogelov, kot so viskoznost, poroznost, sposobnost nabrekanja, degradacija in mehanske lastnosti po odmrznitvi. Potrditev teh hipotez bi prispevala k večji ponovljivosti in natančnosti 3D biotiskljivih in vitro modelov kože.
Ključne besede: in vitro model kože, 3D biotiskanje, hidrogel, predkinična in klinična raziskava
Objavljeno v DKUM: 24.01.2025; Ogledov: 0; Prenosov: 10
Celotno besedilo (2,90 MB)

Opis: V zadnjem času je bil dosežen pomemben napredek pri razvoju ADME (absorpcija, distribucija, metabolizem, ekskrecija) modelov, vendar izziv ostaja vzpostaviti platforme, ki bi zmanjšale testiranje na živalih in stroške raziskav. Naraščajoča pomembnost farmakokinetičnih interakcij poudarja potrebo po zanesljivih in ponovljivih ADME modelih, ki so vse bolj ključni za razvoj zdravil in zagotavljanje varnosti z željo po preprečevanju resnih kliničnih zapletov in hospitalizacije.
Ključne besede: ADME, drug interaction studies, in vitro models, pharmacokinetics, multi-organ models
Objavljeno v DKUM: 07.01.2025; Ogledov: 0; Prenosov: 21
Celotno besedilo (405,16 KB)
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Opis: Despite medical advances, skin-associated disorders continue to pose a unique challenge to physicians worldwide. Skin cancer is one of the most common forms of cancer, with more than one million new cases reported each year. Currently, surgical excision is its primary treatment; however, this can be impractical or even contradictory in certain situations. An interesting potential alternative could lie in topical treatment solutions. The goal of our study was to develop novel multilayer nanofilms consisting of a combination of polyhydroxyethyl methacrylate (PHEMA), polyhydroxypropyl methacrylate (PHPMA), sodium deoxycholate (NaDOC) with incorporated superparamagnetic iron–platinum nanoparticles (FePt NPs), and the potent anticancer drug (5-fluorouracil), for theranostic skin cancer treatment. All multilayer systems were prepared by spin-coating and characterised by atomic force microscopy, infrared spectroscopy, and contact angle measurement. The magnetic properties of the incorporated FePt NPs were evaluated using magnetisation measurement, while their size was determined using transmission electron microscopy (TEM). Drug release performance was tested in vitro, and formulation safety was evaluated on human-skin-derived fibroblasts. Finally, the efficacy for skin cancer treatment was tested on our own basal-cell carcinoma cell line.
Ključne besede: nanomaterials, bimodal therapy, topical skin treatmen, magnetic nanoparticles, thin films, skin cancer, methacrylates
Objavljeno v DKUM: 04.07.2024; Ogledov: 98; Prenosov: 17
Celotno besedilo (5,33 MB)
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Opis: This study presents an innovative wound dressing system that offers a highly effective therapeutic solution for treating painful wounds. By incorporating the widely used non-steroidal anti-inflammatory drug diclofenac, we have created an active wound dressing that can provide targeted pain relief with ease. The drug was embedded within a biocompatible matrix composed of polyhydroxyethyl methacrylate and polyhydroxypropyl methacrylate. The multilayer structure of the dressing, which allows for sustained drug release and an exact application, was achieved through the layer-by-layer coating technique and the inclusion of superparamagnetic iron platinum nanoparticles. The multilayered dressings’ physicochemical, structural, and morphological properties were characterised using various methods. The synergistic effect of the incorporated drug molecules and superparamagnetic nanoparticles on the surface roughness and release kinetics resulted in controlled drug release. In addition, the proposed multilayer wound dressings were found to be biocompatible with human skin fibroblasts. Our findings suggest that the developed wound dressing system can contribute to tailored therapeutic strategies for local pain relief.
Ključne besede: wound dressings, pain relief, superparamagnetic nanoparticles, methacrylate
Objavljeno v DKUM: 19.04.2024; Ogledov: 485; Prenosov: 470
Celotno besedilo (1,61 MB)
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Opis: An electrochemical sensor for the detection of insulin in a single drop (50 μL) was developed based on the concept of molecularly imprinted polymers (MIP). The synthetic MIP receptors were assembled on a screen-printed carbon electrode (SPCE) by the electropolymerization of pyrrole (Py) in the presence of insulin (the protein template) using cyclic voltammetry. After electropolymerization, insulin was removed from the formed polypyrrole (Ppy) matrix to create imprinting cavities for the subsequent analysis of the insulin analyte in test samples. The surface characterization, before and after each electrosynthesis step of the MIP sensors, was performed using atomic force microscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The performance of the developed MIP–SPCE sensor was evaluated using a single drop of solution containing K3Fe(CN)6 and the square-wave voltammetry technique. The MIP–SPCE showed a linear concentration range of 20.0–70.0 pM (R2 = 0.9991), a limit of detection of 1.9 pM, and a limit of quantification of 6.2 pM. The rapid response time to the protein target and the portability of the developed sensor, which is considered a disposable MIP-based system, make this MIP–SPCE sensor a promising candidate for point-of-care applications. In addition, the MIP–SPCE sensor was successfully used to detect insulin in a pharmaceutical sample. The sensor was deemed to be accurate (the average recovery was 108.46%) and precise (the relative standard deviation was 7.23%).
Ključne besede: electrochemical sensors, electropolymerisation, electron microscopy
Objavljeno v DKUM: 14.03.2024; Ogledov: 335; Prenosov: 46
Celotno besedilo (2,91 MB)
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