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Preparation of Three Dimensional Structures of Polysaccharide Derivatives for Application in Regenerative Medicine : doctoral disertation
Andreja Dobaj-Štiglic, 2022, doktorska disertacija

Opis: Biocompatible polysaccharide scaffolds with controllable pore size, good mechanical properties, and no hazardous chemical crosslinkers are desirable for long-term tissue engineering applications. Despite decades of development of novel scaffolds, there are still many challenges to be solved regarding their production and optimization for specifically engineered tissues. Herein, we have fabricated several three-dimensional (3D) scaffolds using polysaccharide or polysaccharide-protein composite hydrogels or inks for 3D printing, featuring strong shear thinning behavior and adequate printability. The inks, composed of various combinations of chitosan, nanofibrillated cellulose, carboxymethyl cellulose, collagen, and citric acid, were 3D printed, freeze-dried, and dehydrothermally heat-treated to obtain dimensionally and mechanically stable scaffolds. The heat-assisted step induced the formation of covalent amide and ester bonds between the functional groups of chosen polysaccharides and protein collagen. Citric acid was chosen as a non-hazardous and „green” crosslinker to further tailor the mechanical properties and long-term stability of the scaffolds. We have investigated how the complexation conditions, charge ratio, dehydrothermal treatment, and degree of crosslinking influence the scaffolds' chemical, surface, swelling, and degradation properties in the dry and hydrated states. The compressive strength, elastic modulus, dimensional stability and shape recovery of the (crosslinked) scaffolds increased significantly with balanced charge ratio, dehydrothermal treatment, and increased concentrations of citric acid crosslinker and collagen concentrations. The prepared crosslinked scaffolds promoted (clustered) cell adhesion and showed no cytotoxic effects, as determined by cell viability assays and live/dead staining with human bone tissue-derived osteoblasts and human adipose tissue-derived mesenchymal stem cells. The water-based and non-hazardous crosslinking methods presented here can be extended to all polysaccharide- or polysaccharide-protein-based materials to develop cell-friendly scaffolds with tailored properties suitable for various tissue engineering applications.
Ključne besede: chitosan, carboxymethyl cellulose, nanofibrillated cellulose, citric acid, collagen, freeze drying, 3D printing, dehydrothermal treatment
Objavljeno v DKUM: 11.10.2022; Ogledov: 127; Prenosov: 34
.pdf Celotno besedilo (33,63 MB)

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Design, Characterisation and Applications of Cellulose-Based Thin Films, Nanofibers and 3D Printed Structures : A Laboratory Manual
Tanja Pivec, Tamilselvan Mohan, Rupert Kargl, Manja Kurečič, Karin Stana-Kleinschek, 2021, drugo učno gradivo

Opis: The introduction of the Laboratory Manual gives the theoretical bases on cellulose and its derivatives, which are used as starting polymers for the preparation of multifunctional polymers with three different advanced techniques - spin coating, electrospinning and 3D printing. In the following, each technique is presented in a separate Lab Exercise. Each exercise covers the theoretical basics on techniques for polymer processing and methods for their characterisation, with an emphasis on the application of prepared materials. The experimental sections contain all the necessary information needed to implement the exercises, while the added results provide students with the help to implement correct and successful exercises and interpret the results.
Ključne besede: multifunctional polymers, polysaccharides, cellulose, electrospun, spin coating, 3D printing, nanofibers, thin films, multifunctional materials, laboratory manuals
Objavljeno v DKUM: 09.03.2021; Ogledov: 468; Prenosov: 20
URL Povezava na datoteko

4.
1st International FibreNet Science for the Public Day : Book of Abstracts
2019, zbornik

Opis: First international conference in the scope of FibreNet project funded by European Commission. The conference brings the FibreNet together international representatives from industry and academia to discuss opportunities and strengths related to bio-based fibre products. In three consecutive sessions, fifteen international PhD students will present their research results, guide you along the value-chain of fibre-based products and give you the opportunity to discuss the future of bio-based materials.
Ključne besede: biobased materials, mechanical properties, paper, packaging, dissemination of research result
Objavljeno v DKUM: 08.01.2020; Ogledov: 843; Prenosov: 40
URL Povezava na datoteko

5.
Photoregeneration of trimethylsilyl cellulose as a tool for microstructuring ultrathin cellulose supports
Archim Wolfberger, Rupert Kargl, Thomas Grießer, Stefan Spirk, 2014, izvirni znanstveni članek

Opis: Microstructured thin films based on cellulose, the most abundant biopolymer on Earth, have been obtained by UV-irradiation of acid-labile trimethylsilyl cellulose thin films in the presence of N-hydroxynaphtalimide triflate as photoacid generator. We demonstrate that this photoregeneration process can be exploited for the manufacture of cellulose patterns having feature sizes down to 1 μm, with potential applications in life sciences.
Ključne besede: cellulose thin films, cellulose regeneration, patterning of cellulose, photoregeneration
Objavljeno v DKUM: 22.06.2017; Ogledov: 1040; Prenosov: 408
.pdf Celotno besedilo (886,41 KB)
Gradivo ima več datotek! Več...

6.
Book of abstracts : Future Perspectives in Polysaccharide Research
2015, druge monografije in druga zaključena dela

Opis: 1st International EPNOE Junior Scientists Meeting "Future Perspectives in Polysaccharide Research", 19-20th January 2015
Objavljeno v DKUM: 14.09.2016; Ogledov: 899; Prenosov: 50
.pdf Celotno besedilo (543,63 KB)

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Book of abstracts
2015, druge monografije in druga zaključena dela

Objavljeno v DKUM: 30.12.2015; Ogledov: 421; Prenosov: 14
URL Povezava na celotno besedilo

8.
Application and characterization of microstructured cellulose thin films
Rupert Kargl, Tamilselvan Mohan, Aleš Doliška, Karin Stana-Kleinschek, Volker Ribitsch, 2013, objavljeni znanstveni prispevek na konferenci

Ključne besede: celuloza
Objavljeno v DKUM: 10.07.2015; Ogledov: 1375; Prenosov: 19
URL Povezava na celotno besedilo

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