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1.
Description of Komagataeibacter melaceti sp. nov. and Komagataeibacter melomenusus sp. nov. isolated from apple cider vinegar
Leon Marič, Ilse Cleenwerck, Tomaž Accetto, Peter Vandamme, Janja Trček, 2020, izvirni znanstveni članek

Opis: Two novel strains AV382 and AV436 were isolated from a submerged industrial bioreactor for production of apple cider vinegar in Kopivnik (Slovenia). Both strains showed very high (≥98.2%) 16S rRNA gene sequence similarities with Komagataeibacter species, but lower 16S–23S rRNA gene internal transcribed spacer (ITS). The highest similarity of the 16S–23S rRNA gene ITS of AV382 was to Komagataeibacter kakiaceti LMG 26206$^T$ (91.6%), of AV436 to Komagataeibacter xylinus LMG 1515$^T$ (93.9%). The analysis of genome sequences confirmed that AV382 is the most closely related to K. kakiaceti (ANIb 88.2%) and AV436 to K. xylinus (ANIb 91.6%). Genome to genome distance calculations exhibit for both strains ≤47.3% similarity to all type strains of the genus Komagataeibacter. The strain AV382 can be differentiated from its closest relatives K. kakiaceti and Komagataeibacter saccharivorans by its ability to form 2-keto and 5-keto-D-gluconic acids from glucose, incapability to grow in the presence of 30% glucose, formation of C$_{19:0}$ cyclo ω8c fatty acid and tolerance of up to 5% acetic acid in the presence of ethanol. The strain AV436 can be differentiated from its closest relatives K. xylinus, Komagataeibacter sucrofermentans, and Komagataeibacter nataicola by its ability to form 5-keto-D-gluconic acid, growth on 1-propanol, efficient synthesis of cellulose, and tolerance to up to 5% acetic acid in the presence ethanol. The major fatty acid of both strains is C$_{18:1}$ ω7c. Based on a combination of phenotypic, chemotaxonomic and phylogenetic features, the strains AV382$^T$ and AV436$^T$ represent novel species of the genus Komagataeibacter, for which the names Komagataeibacter melaceti sp. nov. and Komagataeibacter melomenusus are proposed, respectively. The type strain of Komagataeibacter melaceti is AV382$^T$ (= ZIM B1054$^T$ = LMG 31303$^T$ = CCM 8958$^T$) and of Komagataeibacter melomenusus AV436$^T$ (= ZIM B1056$^T$ = LMG 31304$^T$ = CCM 8959$^T$).
Ključne besede: Acetic acid bacteria, Acetobacteraceae, Komagataeibacter, Komagataeibacter melaceti, Komagataeibacter melomenusus, vinegar
Objavljeno v DKUM: 02.09.2022; Ogledov: 80; Prenosov: 4
URL Povezava na celotno besedilo

2.
Acetan and acetan-like polysaccharides: genetics, biosynthesis, structure, and viscoelasticity
Janja Trček, Iztok Dogša, Tomaž Accetto, David Stopar, 2021, pregledni znanstveni članek

Opis: Bacteria produce a variety of multifunctional polysaccharides, including structural, intracellular, and extracellular polysaccharides. They are attractive for the industrial sector due to their natural origin, sustainability, biodegradability, low toxicity, stability, unique viscoelastic properties, stable cost, and supply. When incorporated into different matrices, they may control emulsification, stabilization, crystallization, water release, and encapsulation. Acetan is an important extracellular water-soluble polysaccharide produced mainly by bacterial species of the genera Komagataeibacter and Acetobacter. Since its original description in Komagataeibacter xylinus, acetan-like polysaccharides have also been described in other species of acetic acid bacteria. Our knowledge on chemical composition of different acetan-like polysaccharides, their viscoelasticity, and the genetic basis for their production has expanded during the last years. Here, we review data on acetan biosynthesis, its molecular structure, genetic organization, and mechanical properties. In addition, we have performed an extended bioinformatic analysis on acetan-like polysaccharide genetic clusters in the genomes of Komagataeibacter and Acetobacter species. The analysis revealed for the first time a second acetan-like polysaccharide genetic cluster, that is widespread in both genera. All species of the Komagataeibacter possess at least one acetan genetic cluster, while it is present in only one third of the Acetobacter species surveyed.
Ključne besede: extracellular polysaccharide, acetan, acetan genetic cluster, acetan synthesis, acetan viscoelasticity, applications of acetan, Komagataeibacter, Acetobacter
Objavljeno v DKUM: 02.09.2022; Ogledov: 61; Prenosov: 3
URL Povezava na celotno besedilo

3.
Mnenje slovenskih žensk o izvajanju preventivnih babiških pregledov v nosečnosti
Špela Accetto, 2020, magistrsko delo

Opis: Babica je usposobljena za spremljanje fiziološke nosečnosti in po zakonskih predpisih je pregled v 16., 32., 37., 38. in 39. tednu nosečnosti njena kompetenca. Kljub temu ženske v slovenskem zdravstvenem sistemu v večji meri preglede opravljajo pri izbranem ginekologu/porodničarju.V zaključnem delu smo želeli ugotoviti, koliko slovenske ženske poznajo babiške kompetence in kakšno je njihovo mnenje glede izvajanja preventivnih babiških pregledov v nosečnosti.
Ključne besede: babištvo, kompetence, preporodna oskrba, reproduktivno zdravje, promocija zdravja.
Objavljeno v DKUM: 11.03.2020; Ogledov: 968; Prenosov: 153
.pdf Celotno besedilo (1,55 MB)

4.
Discrete event simulation of administrative and medical processes
Robert Leskovar, Rok Accetto, Alenka Baggia, Zlatko Lazarevič, Goran Vukovič, Peter Požun, 2011, pregledni znanstveni članek

Opis: Background: Medical processes are often obstructed by administrative ones. The main issue in administrative processes is uneven workload resulting in an increased possibility of human errors. The system approach assures that medical and administrative processes are integrated. According to research reports and best practices, discrete event simulation is a proper method to implement the system approach. Methods: A detailed analysis of the administrative processes was performed using interviews, UML diagrams and flowcharts. Based on the data gathered from the information system and measurements on the site, the distribution of patient arrivals and service times were modelled. The aim of discrete event simulation models was to replicate the behaviour of the existing system (separate administration) and to simulate the changes proposed (joint administration). Results: Average utilizations of administrative personnel in 100 simulation runs for specific clinical departments are: 83.8 % at the Department of Rheumatology, 61.9 % at the Department of Hypertension, and 47.2 % at the Veteransć Medical Unit. Should joint administration be applied, the average utilization of administrative personnel would be 74.3%. Conclusions: Discrete event simulation proved that joint administration would contribute to a more even workload distribution among administrative personnel, higher quality of service and easier human resource management. The presented approach can be efficiently applied to large-scale systems e.g. organizational changes of processes in Specialist Outpatient Clinics.
Ključne besede: health care, discrete event simulation, quality of service
Objavljeno v DKUM: 27.03.2017; Ogledov: 895; Prenosov: 130
.pdf Celotno besedilo (702,94 KB)
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