Iskanje po katalogu digitalne knjižnice

Opis: The preconditioning and modification of the supporting electrode surface is an essential step in every biosensor architecture. In particular, when using screen-printed carbon electrodes (SPEs) as inexpensive and convenient disposable sensor substrates, their somewhat lower electrochemical (surface) reproducibility might represent a complex hurdle. Herein, we investigated the effect of selected preconditioning strategies, such as cyclic voltammetric pretreatment, in H2SO4 and H2O2 and plasma pretreatment with a positive and negative glow discharge, which all improved the electrochemical stability of the unmodified SPEs. Furthermore, we studied the influence of preconditioning strategies on the adsorption kinetics of the two most commonly used building blocks for biosensor preparation, i.e., bovine serum albumin (BSA) and protein A. We observed an advantageous effect of all the examined preconditioning strategies for the modification of SPEs with protein A, being the most effective the negative glow discharge. On the other hand, BSA exhibited a more complex adsorption behavior, with the negative glow discharge as the only generally beneficial preconditioning strategy providing the highest electrochemical stability. Protein A revealed a more substantial impact on the electrochemical signal attenuation than BSA considering their same concentrations in the modification solutions. For both BSA and protein A, we showed that the concentrations of 5 and 10 µg mL−1 already suffice for an electrochemically satisfactorily stable electrode surface after 60 min of incubation time, except for BSA at the positive-plasma-treated electrode.
Ključne besede: screen-printed carbon electrode, SPE, electrochemical biosensor, adsorption, bovine serum albumin, protein A, glow discharge
Objavljeno v DKUM: 15.05.2025; Ogledov: 0; Prenosov: 2
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Opis: Differences in adipose tissue deposition and properties between pig male sex categories, i.e., entire males (EM), immunocastrates (IC) and surgical castrates (SC) are relatively well-characterized, whereas the underlying molecular mechanisms are still not fully understood. To gain knowledge about the genetic regulation of the differences in adipose tissue deposition, two different approaches were used: RNA-sequencing and candidate gene expression by quantitative PCR. A total of 83 differentially expressed genes were identified between EM and IC, 15 between IC and SC and 48 between EM and SC by RNA-sequencing of the subcutaneous adipose tissue. Comparing EM with IC or SC, upregulated genes related to extracellular matrix dynamics and adipogenesis, and downregulated genes involved in the control of lipid and carbohydrate metabolism were detected. Differential gene expression generally indicated high similarity between IC and SC as opposed to EM, except for several heat shock protein genes that were upregulated in EM and IC compared with SC. The candidate gene expression approach showed that genes involved in lipogenesis were downregulated in EM compared with IC pigs, further confirming RNA-sequencing results.
Ključne besede: pigs, adipose tissue, entire males, immunocastration, surgical castration, RNA-sequencing, expression
Objavljeno v DKUM: 10.10.2024; Ogledov: 0; Prenosov: 5
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Opis: Background: While immunocastration has been studied in male pre-pubertal pigs, data on older, sexually mature animals are limited. To understand the physiological effects of androgen deprivation in the late sexual development phase, we compared mature immunocastrated boars (n = 19; average age = 480 days) to young male immunocastrated pigs (n = 6; average age = 183 days) and young entire males (n = 6; average age = 186 days) as positive and negative controls, respectively. Objectives: We hypothesized that the timing of gonadotropin-releasing hormone suppression (early or late sexual development phases) influences the extent of reproductive function inhibition, histological structure of testicular tissue, and expression levels of selected genes related to steroid metabolism. Materials and methods: Antibody titer, hormonal status, and histomorphometric analysis of testicular tissue were subjected to principal component analysis followed by hierarchical clustering to evaluate the immunocastration effectiveness in mature boars. Results: Hierarchical clustering differentiated mature immunocastrated boars clustered with young immunocastrated pigs from those clustered with entire males. Although all mature immunocastrated boars responded to vaccination, as evidenced by the increased gonadotropin-releasing hormone antibody titers (p < 0.001), decreased serum luteinizing hormone concentrations (p = 0.002), and changes in testicular tissue vascularization (lighter and less red testicular parenchyma; p ≤ 0.001), the responses were variable. Sharp decreases in testes index (p < 0.001), Leydig cell volume density (p < 0.001), Leydig cell nucleus-to-cytoplasm ratio (p < 0.001), and testosterone concentration (p < 0.001) were observed in mature immunocastrated boars clustered with young immunocastrated pigs compared with those that clustered with entire males. Additionally, mature immunocastrated boars clustered with young immunocastrated pigs showed lower hydroxysteroid 17-beta dehydrogenase 7 expression than entire males (p < 0.05). The young immunocastrated pigs group showed higher folliclestimulating hormone receptors than the entire males and mature immunocastrated boars, lower steroidogenic acute regulatory protein expression levels compared with entire males, and mature immunocastrated boars clustered with entire males (p < 0.01). Conclusion: The two-dose vaccination regime resulted in progressive but variable regression of testicular function in adult (post-pubertal) pigs; however, it was insufficient to induce a complete immunocastration response in all animals.
Ključne besede: response to immunocastration, pigs, anti-GnRH, morphometry, testicular mRNA expression
Objavljeno v DKUM: 16.08.2023; Ogledov: 490; Prenosov: 104
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Opis: The influence of the immunocastration (immunisation against GnRH) on pig performance (growth, carcass and meat quality traits) was investigated in two parallel experiments (on two farms) with two crossbreeds – G1 (50% Duroc) and G2 (50% Pietrain). Within the crossbreed, the pigs were assigned to three experimental groups; entire males (EM, n=49), immunocastrates (IC, n=45) and surgical castrates (SC, n=45). Those assigned to IC group were vaccinated at the age of 12 and 19 weeks. Pigs were individually housed, their feed intake (ad libitum) and weight (at 12, 19 and 24 weeks) were recorded. At the age of 24 weeks, the pigs were slaughtered and their carcass and meat quality traits were assessed. We hypothesized that treatment response could have been different in two crossbreeds. However the interaction was insignificant, thus the treatment effect is presented on pooled results for both crossbreeds. Until the revaccination, IC were similar to EM pigs, thereafter they exhibited an increase in feed intake and growth rate. Overall, they presented an advantage in growth rate and feed efficiency as compared to SC. They also exhibited better carcass properties as SC without any major effect on meat quality. The present study provides the initial information on the immunocastration effect in Slovenian herds that should further be supported by testing it in usual rearing conditions and group housing.
Ključne besede: pig, immunocastration, growth performance, carcass properties, meat quality
Objavljeno v DKUM: 24.08.2017; Ogledov: 1983; Prenosov: 111
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