1. Protocol for the fabrication of self-standing (nano)cellulose-based 3D scaffolds for tissue engineeringTamilselvan Mohan, Matej Bračič, Doris Bračič, Florian Lackner, Chandran Nagaraj, Andreja Dobaj-Štiglic, Rupert Kargl, Karin Stana-Kleinschek, 2025, izvirni znanstveni članek Opis: Three-dimensional (3D) and porous scaffolds made from nanocellulosic materials hold significant potential in tissue engineering (TE). Here, we present a protocol for fabricating self-standing (nano)cellulose-based 3D scaffolds designed for in vitro testing of cells from skin and cartilage tissues. We describe steps for preparation of nanocellulose ink, scaffold formation using 3D printing, and freeze-drying. We then detail post-processing procedures to enhance mechanical properties, stability, and biocompatibility. This protocol offers researchers a framework for developing versatile and sustainable biomaterials for regenerative medicine.
Ključne besede: tissue engineering, 3D scaffolds, nanocellulosic materials, biocompatibility
Objavljeno v DKUM: 03.11.2025; Ogledov: 0; Prenosov: 4
 Celotno besedilo (4,46 MB)
Gradivo ima več datotek! Več... |
2. Chemo-enzymatic synthesis of viscoelastic pseudopeptidoglycan networksPhilipp Loibner, David Bučak-Gasser, Katharina Schober, Tobias Alexander Steindorfer, Monika Brandtner, Tobias Dorn, Tanja Wrodnigg, Dmytro Neshchadin, Georg Gescheidt-Demner, Matej Bračič, Florian Lackner, Tamilselvan Mohan, Karin Stana-Kleinschek, Rupert Kargl, 2025, izvirni znanstveni članek Opis: Bacterial peptidoglycans (PGs) are essential targets for antibiotics and immune cells. Chemical methods to reproduce PGs semisynthetically are tedious and wasteful. In this work, we describe a new approach to form pseudo-PGs (PPGs) using the protease papain and custom-made peptides conjugated to a glycan. The kinetics of formation is monitored by rheology and 1H NMR. Viscoelastic gels of controlled strength are formed, depending on the temperature and the number of peptide bridges between the glycan chains. We propose that the new method has a high impact on biomaterials research, since it could be used to deliver peptides, test antibiotic efficacy, or investigate human immune cell response.
Ključne besede: biomaterials, bacterial peptidoglycans (PGs), chemo-enzymatic synthesis
Objavljeno v DKUM: 03.11.2025; Ogledov: 0; Prenosov: 3
Celotno besedilo (4,32 MB)
Gradivo ima več datotek! Več... |
3. Functionalization of polycaprolactone 3D scaffolds with hyaluronic acid glycine-peptide conjugates and endothelial cell adhesionTamilselvan Mohan, Fazilet Gürer, Doris Bračič, Florian Lackner, Chandran Nagaraj, Uroš Maver, Lidija Gradišnik, Matjaž Finšgar, Rupert Kargl, Karin Stana-Kleinschek, 2025, izvirni znanstveni članek Opis: This study enhances the bioactivity of polycaprolactone (PCL) scaffolds for tissue engineering by functionalizing them with oxidized hyaluronic acid glycine-peptide conjugates to improve endothelial cell adhesion and growth. Hyaluronic acid was conjugated with a glycine-peptide to create a bioactive interface on PCL (static water contact angle, SCA(H2O): 98°). The scaffolds were fabricated using a melt extrusion 3D printing technique. The HA-glycine peptide conjugates were oxidized and immobilized on aminolyzed PCL via Schiff-base chemistry, introducing hydrophilicity (SCA(H2O): 21°), multiple functional groups, and a negative zeta potential (-12.04 mV at pH 7.4). A quartz crystal microbalance confirmed chemical conjugation and quantified the mass (8.5-10.3 mg m-2) of oxidized HA-glycine on PCL. The functionalized scaffolds showed enhanced swelling, improved mechanical properties (2-fold increase in strength, from 26 to 51 MPa), and maintained integrity during degradation. In-vitro experiments demonstrated improved endothelial cell adhesion, proliferation and viability, suggesting the potential for vascularized tissue constructs.
Ključne besede: 3D printing, polycaprolactone, hyaluronic acid
Objavljeno v DKUM: 19.03.2025; Ogledov: 0; Prenosov: 3
Celotno besedilo (9,50 MB)
Gradivo ima več datotek! Več... |
4. Antithrombogenic polysaccharide coatings to improve hemocompatibility, protein-repellence, and endothelial cell responseMatej Bračič, Bence M. Nagy, Olivija Plohl, Florian Lackner, Tobias Alexander Steindorfer, Roland Fischer, Thomas Heinze, Andrea Olschewski, Karin Stana-Kleinschek, Chandran Nagaraj, Tamilselvan Mohan, 2024, izvirni znanstveni članek Opis: Polyester biomaterials play a crucial in vascular surgery, but suffer from unspecific protein adsorption,
thrombogenicity, and inadequate endothelial cell response, which limit their success. To address these issues, we investigated the functionalization of polyester biomaterials with antithrombogenic polysaccharide coatings. A two-step and water-based method was used to coat cationized polycaprolactone with
different sulfated polysaccharides (SPS), which resulted in long-term stability, tunable morphology,
roughness, film thickness, chemical compositions, zeta potential, and water content. The coatings significantly increased the anticoagulant activity and reduced the thrombogenicity of polycaprolactone, particularly with highly sulfated heparin and cellulose sulfate. Less SPS, such as chondroitin sulfate, fucoidan,
and carrageenan, despite showing reduced anticoagulant activity, also exhibited lower fibrinogen adsorption. The adhesion and viability of human primary endothelial cells cultured on modified polycaprolactone
correlated with the type and sulfate content of the coatings.
Ključne besede: polyester biomaterials, vascular surgery, antithrombogenic polysaccharide coatings
Objavljeno v DKUM: 10.09.2024; Ogledov: 65; Prenosov: 22
Celotno besedilo (5,07 MB)
Gradivo ima več datotek! Več... |
5. 3D-printed anisotropic nanofiber composites with gradual mechanical propertiesFlorian Lackner, Ivan Knechtl, Maximilian Novak, Chandran Nagaraj, Andreja Dobaj-Štiglic, Rupert Kargl, Andrea Olschewski, Karin Stana-Kleinschek, Tamilselvan Mohan, 2023, izvirni znanstveni članek Ključne besede: 3D printing, anisotropy nanocomposites, biomedical applications, mechanical properties, nanocellulose alginate
Objavljeno v DKUM: 03.06.2024; Ogledov: 132; Prenosov: 18
Celotno besedilo (4,26 MB)
Gradivo ima več datotek! Več... |
6. 3D-printed collagen–nanocellulose hybrid bioscaffolds with tailored properties for tissue engineering applicationsAndreja Dobaj-Štiglic, Florian Lackner, Chandran Nagaraj, Marco Beaumont, Matej Bračič, Isabel Duarte, Veno Kononenko, Damjana Drobne, Balaraman Madhan, Matjaž Finšgar, Rupert Kargl, Karin Stana-Kleinschek, Tamilselvan Mohan, 2023, izvirni znanstveni članek Ključne besede: assays, degradation, porosity, scaffolds, 3D printing
Objavljeno v DKUM: 09.04.2024; Ogledov: 238; Prenosov: 810
Celotno besedilo (9,95 MB)
Gradivo ima več datotek! Več... |
