1. Functionalization of polycaprolactone 3D scaffolds with hyaluronic acid glycine-peptide conjugates and endothelial cell adhesionTamilselvan Mohan, Fazilet Gürer, Doris Bračič, Florian Lackner, Chandran Nagaraj, Uroš Maver, Lidija Gradišnik, Matjaž Finšgar, Rupert Kargl, Karin Stana-Kleinschek, 2025, izvirni znanstveni članek Opis: This study enhances the bioactivity of polycaprolactone (PCL) scaffolds for tissue engineering by functionalizing them with oxidized hyaluronic acid glycine-peptide conjugates to improve endothelial cell adhesion and growth. Hyaluronic acid was conjugated with a glycine-peptide to create a bioactive interface on PCL (static water contact angle, SCA(H2O): 98°). The scaffolds were fabricated using a melt extrusion 3D printing technique. The HA-glycine peptide conjugates were oxidized and immobilized on aminolyzed PCL via Schiff-base chemistry, introducing hydrophilicity (SCA(H2O): 21°), multiple functional groups, and a negative zeta potential (-12.04 mV at pH 7.4). A quartz crystal microbalance confirmed chemical conjugation and quantified the mass (8.5-10.3 mg m-2) of oxidized HA-glycine on PCL. The functionalized scaffolds showed enhanced swelling, improved mechanical properties (2-fold increase in strength, from 26 to 51 MPa), and maintained integrity during degradation. In-vitro experiments demonstrated improved endothelial cell adhesion, proliferation and viability, suggesting the potential for vascularized tissue constructs.
Ključne besede: 3D printing, polycaprolactone, hyaluronic acid
Objavljeno v DKUM: 19.03.2025; Ogledov: 0; Prenosov: 3
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2. Antithrombogenic polysaccharide coatings to improve hemocompatibility, protein-repellence, and endothelial cell responseMatej Bračič, Bence M. Nagy, Olivija Plohl, Florian Lackner, Tobias Alexander Steindorfer, Roland Fischer, Thomas Heinze, Andrea Olschewski, Karin Stana-Kleinschek, Chandran Nagaraj, Tamilselvan Mohan, 2024, izvirni znanstveni članek Opis: Polyester biomaterials play a crucial in vascular surgery, but suffer from unspecific protein adsorption,
thrombogenicity, and inadequate endothelial cell response, which limit their success. To address these issues, we investigated the functionalization of polyester biomaterials with antithrombogenic polysaccharide coatings. A two-step and water-based method was used to coat cationized polycaprolactone with
different sulfated polysaccharides (SPS), which resulted in long-term stability, tunable morphology,
roughness, film thickness, chemical compositions, zeta potential, and water content. The coatings significantly increased the anticoagulant activity and reduced the thrombogenicity of polycaprolactone, particularly with highly sulfated heparin and cellulose sulfate. Less SPS, such as chondroitin sulfate, fucoidan,
and carrageenan, despite showing reduced anticoagulant activity, also exhibited lower fibrinogen adsorption. The adhesion and viability of human primary endothelial cells cultured on modified polycaprolactone
correlated with the type and sulfate content of the coatings.
Ključne besede: polyester biomaterials, vascular surgery, antithrombogenic polysaccharide coatings
Objavljeno v DKUM: 10.09.2024; Ogledov: 65; Prenosov: 10
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3. 3D-printed anisotropic nanofiber composites with gradual mechanical propertiesFlorian Lackner, Ivan Knechtl, Maximilian Novak, Chandran Nagaraj, Andreja Dobaj-Štiglic, Rupert Kargl, Andrea Olschewski, Karin Stana-Kleinschek, Tamilselvan Mohan, 2023, izvirni znanstveni članek Ključne besede: 3D printing, anisotropy nanocomposites, biomedical applications, mechanical properties, nanocellulose alginate
Objavljeno v DKUM: 03.06.2024; Ogledov: 132; Prenosov: 14
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4. 3D-printed collagen–nanocellulose hybrid bioscaffolds with tailored properties for tissue engineering applicationsAndreja Dobaj-Štiglic, Florian Lackner, Chandran Nagaraj, Marco Beaumont, Matej Bračič, Isabel Duarte, Veno Kononenko, Damjana Drobne, Balaraman Madhan, Matjaž Finšgar, Rupert Kargl, Karin Stana-Kleinschek, Tamilselvan Mohan, 2023, izvirni znanstveni članek Ključne besede: assays, degradation, porosity, scaffolds, 3D printing
Objavljeno v DKUM: 09.04.2024; Ogledov: 238; Prenosov: 799
Celotno besedilo (9,95 MB)
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