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Morphology, aggregation properties, cytocompatibility and anti-inflammatory potential of citrate-stabilized AuNPs prepared by modular Ultrasonic Spray Pyrolysis
Rebeka Rudolf, Peter Majerič, Sergej Tomić, Mohammed Shariq, Urban Ferčec, Bojan Budič, Bernd Friedrich, Dragana Vučević, Miodrag Čolić, 2017, izvirni znanstveni članek

Opis: Ultrasonic Spray Pyrolysis (USP) possesses a great potential for production of higher quantities of gold nanoparticles (AuNPs), thus overcoming the problem of batch-to-batch variations in their properties. Recently, we demonstrated that USP with an additional evaporation chamber (modular USP) led to a better size control of AuNPs. However, their morphology, stability, toxicity, and immunomodulatory properties have not been investigated completely. Here, two types of spherical AuNPs were produced by using different USP parameters, followed by their stabilization in Na-citrate solution. No significant changes in their size, agglomeration, and -potential occurred 3 months after their initial production in citrate solution. However, the conditioning of AuNPs in serum-containing cell culture media for 24 h induced an increase in the AuNPs’ hydrodynamic size and a red shift in their Surface Plasmon Resonance, pointing to their instability in biological media. Cytocompatibility tests showed that the produced AuNPs were internalized by L929 cells and primary human monocytes and were not cytotoxic at the concentrations lower than 200 μg/mL, but they exhibited antiproliferative and anti-inflammatory effects, respectively. AuNPs reduced the percentage of CD14+CD16+ but not CD14lowCD16+ monocytes in vitro and reduced the expression of CD86, HLA-DR, TNF-α, and IL-12/IL-23 by these cells. These results indicate that the anti-inflammatory effects of citrate-capped AuNPs produced by modular USP could be beneficial for their application in the treatment of inflammatory conditions.
Ključne besede: Ultrasonic Spray Pyrolysis, gold nanoparticles, plasmon resonance, monocytes, antiinflammatory effects
Objavljeno: 14.06.2017; Ogledov: 236; Prenosov: 33
.pdf Celotno besedilo (5,41 MB)

Size-dependent effects of gold nanoparticles uptake on maturation and antitumor functions of human dendritic cells in vitro
Sergej Tomić, Nina Ogrinc Potočnik, Rebeka Rudolf, Primož Pelicon, Ivan Anžel, Marjan Rupnik, Jelena Đokić, Saša Vasilijić, Dragana Vučević, Petar Milosavljević, Srđa Janković, Jelena Rajković, Bernd Friedrich, Miodrag Čolić, 2014, izvirni znanstveni članek

Opis: Gold nanoparticles (GNPs) are claimed as outstanding biomedical tools for cancer diagnostics and photo-thermal therapy, but without enough evidence on their potentially adverse immunological effects. Using a model of human dendritic cells (DCs), we showed that 10 nm- and 50 nm-sized GNPs (GNP10 and GNP50, respectively) were internalized predominantly via dynamin-dependent mechanisms, and they both impaired LPS-induced maturation and allostimulatory capacity of DCs, although the effect of GNP10 was more prominent. However, GNP10 inhibited LPS-induced production of IL-12p70 by DCs, and potentiated their Th2 polarization capacity, while GNP50 promoted Th17 polarization. Such effects of GNP10 correlated with a stronger inhibition of LPS-induced changes in Ca2+ oscillations, their higher number per DC, and more frequent extraendosomal localization, as judged by live-cell imaging, proton, and electron microscopy, respectively. Even when released from heat-killed necrotic HEp-2 cells, GNP10 inhibited the necrotic tumor cell-induced maturation and functions of DCs, potentiated their Th2/Th17 polarization capacity, and thus, impaired the DCs% capacity to induce T cell-mediated anti-tumor cytotoxicity in vitro. Therefore, GNP10 could potentially induce more adverse DC-mediated immunological effects, compared to GNP50.
Ključne besede: gold, nanoparticles, antitumor function
Objavljeno: 19.06.2017; Ogledov: 245; Prenosov: 33
.pdf Celotno besedilo (5,60 MB)

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