1. The efficacy of probiotics as antiviral agents for the treatment of rotavirus gastrointestinal infections in children : an updated overview of literatureAndrej Steyer, Dušanka Mičetić-Turk, Sabina Fijan, 2022, pregledni znanstveni članek Opis: Enteric viruses, including the rotavirus, norovirus, and adenoviruses, are the most common cause of acute gastroenteritis. The rotavirus disease is especially prevalent among children, and studies over the past decade have revealed complex interactions between rotaviruses and the gut microbiota. One way to treat and prevent dysbiosis is the use of probiotics as an antiviral agent. This review focuses on the latest scientific evidence on the antiviral properties of probiotics against rotavirus gastroenteric infections in children. A total of 19 studies exhibited a statistically significant antiviral effect of probiotics. The main probiotics that were effective were Saccharomyces cerevisiae var. boulardii, Lacticaseibacillus rhamnosus GG, and various multi-strain probiotics. The underlying mechanism of the probiotics against rotavirus gastroenteric infections in children included immune enhancement and modulation of intestinal microbiota leading to shortening of diarrhoea. However, several clinical studies also found no significant difference in the probiotic group compared to the placebo group even though well-known strains were used, thus showing the importance of correct dosage, duration of treatment, quality of probiotics and the possible influence of other factors, such as the production process of probiotics and the influence of immunisation on the effect of probiotics. Therefore, more robust, well-designed clinical studies addressing all factors are warranted.
Ključne besede: probiotics, microbiota, rotaviruses
Objavljeno v DKUM: 05.07.2024; Ogledov: 95; Prenosov: 11
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2. Primerjava med izbranimi mikroorganizmi: probiotični in patogeni seviMojca Fifer, 2023, magistrsko delo Opis: Uvod: Mikroorganizmi so vseživljensko pomembni in prisotni v našem življenju. Poznamo patogene, komenzalne in oportunistične seve mikroorganizmov. Mnogi sevi mikroorganizmov so tudi probiotiki. Zaradi obširnosti mikroorganizmov smo se osredotočili samo na rodove Enterococcus, Streptococcus, Bacillus, Clostridioides, Escherichia in Klebsiella. Postavili smo si raziskovalna vprašanja, ki so se nam zdela ključna pri raziskovanju patogenih in probiotičnih sevov mikroorganizmov.
Metode: S sistematičnim pregledom strokovne domače in tuje literature smo iskali probiotične in patogene mikroorganizme. Uporabili smo metodo PRISMA in v obliki tabel prikazali rezultate. Na podlagi pregleda literature smo po bazah podatkov pridobili 18.260 člankov, odstranili duplikate in članke analizirali glede ustreznosti. V magistrski nalogi smo vključili 62 preglednih in izvirno znanstvenih člankov, ki so vsebinsko ustrezali naši raziskovani temi.
Rezultati: Ugotovili smo, da obstajajo iste taksonomsko vrste, ki imajo koristne in škodljive značilnosti. Probiotične seve uporabljajo pri zdravljenju bolezni, ki jih ob okužbah povzročajo patogeni sevi. Probiotični in patogeni sevi se razlikujejo v virulenci, toksičnosti, odpornosti na okolje z ekstremnimi pH vrednostmi, odpornosti proti antibiotikom in metabolitih.
Razprava in sklep: Na podlagi rezultatov ugotavljamo, da ne moremo kategorizirat taksonomskih vrst na patogene ali probiotične mikroorganizme, zato je potrebno nujno poznavanje specifičnih lastnosti posameznih sevov mikroorganizma.
Ključne besede: Bacillus, Escherichia, Klebsiella, Lactobacillus, Clostridioides
Objavljeno v DKUM: 14.12.2023; Ogledov: 387; Prenosov: 64
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3. Vpliv različnih probiotikov na rast in preživetje bakterije Escherichia coli v mlekuDunja Šulc, 2017, magistrsko delo Opis: Probiotiki so živi mikroorganizmi, ki dokazano pozitivno učinkujejo na zdravje gostitelja, če jih zaužije v zadostnih količinah. Probiotični sevi imajo različne mehanizme delovanja in opravljajo specifične naloge. Na trgu je dostopnih vedno več probiotičnih izdelkov, predvsem v obliki prehranskih dopolnil. Zaradi potrošniške industrije moramo biti pozorni, da za probiotike štejemo zgolj izdelke, ki so analizirani in vsebujejo probiotične seve, katerih učinke potrjujejo klinične raziskave. V magistrskem delu smo preverili vpliv različnih probiotikov na rast in preživetje bakterije Escherichia coli v mleku.
Ključne besede: probiotiki, pozitivni učinki probiotikov, Escherichia coli, klasične gojitvene metode štetja mikroorganizmov, PCR, metoda agar spot.
Objavljeno v DKUM: 18.01.2018; Ogledov: 2345; Prenosov: 126
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4. Rotaviral RNA found on various surfaces in a hospital laundrySabina Fijan, Andrej Steyer, Mateja Poljšak-Prijatelj, Avrelija Cencič, Sonja Šostar-Turk, Srečko Koren, 2008, izvirni znanstveni članek Opis: The aim of this investigative study was to determine the presence of rotaviral RNA at various control points (CP) of a hospital laundry. One of the possible sources of hospital infections is inappropriately laundered and disinfected hospital textiles. RT-PCR and nested PCR for gene amplification using specific primers following RNA isolation were used to determine the presence of rotaviral RNA on swabs. In addition, rotavirus suspensions were inoculated on marked surfaces as positive controls for different surfaces (cotton textiles, folding table and industrial dryer). Rotaviral RNA was found on various laundry surfaces: technical equipment, storage shelves, transport vehicles, personnel's hands, damp textiles, and folded laundry. Rotaviral RNA was also detected at all positive controls on tested surfaces after 24 h. Based on the results, it is very important to take into consideration the proper handling of textiles after washing as one of the precautions against hospital-acquired infections. This paper reports the presence of rotaviral RNA for the first time on surfaces in laundries and equipment, as well as textiles.
Ključne besede: laundry hygiene, rotaviruses, hospital-acquired disease prevention, sanitary measures, occupational health
Objavljeno v DKUM: 01.06.2012; Ogledov: 2858; Prenosov: 96
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