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Opis: The interaction of microorganisms and their enzyme activity is one of the key indicators for a comprehensive measurement of soil health. The aim of this study was to determine significant correlations between different soil microorganisms and enzyme activities of β-glucosidase, N-acetyl-glucosaminidase, urease, arylamidase, phosphatase, acid phosphatase, alkaline phosphatase, and arylsulfatase after supplementation with standard fertilizer, spent mushroom substrate and composed fertilizer in soils from conventional–integrated, organic and biodynamic farming. Samples were grouped according to the farming system and fertilization for all seasons. The biodynamic farm was the least affected by the different fertilizations, except for standard fertilization. Standard fertilizer caused negative correlations between the actinomycetes and the arylsulfatase in organic and biodynamic farms. The same fertilization affected the actinomycetes/phosphatase relationship differently, regardless of the basic soil structure. Actinomycetes correlated positively with acid phosphatase and urease in conventional–integrated and biodynamic farms after spent mushroom substrate, respectively. Arylamidase activity in relation to total microorganisms responded to fertilization with standard fertilizer and spent mushroom substrate independently of the basic soil structure. Fertilization can influence the soil microbe/enzyme relationships in different soils. Regardless of the basic soil structure, some of these relationships could be important indicators for further studies.
Ključne besede: agriculture, conventional-integrated, organic, biodynamic, fertilization, soil microorganisms, soil enzyme activity
Objavljeno v DKUM: 17.04.2025; Ogledov: 0; Prenosov: 3
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Opis: Izrabljen substrat za gobe (IGS) je stranski proizvod gojenja gob, ki sestoji iz raznovrstnih organskih materialov. Po nekaj sezonah rabe se substrat izčrpa in praviloma velja za odpadek, vendar sodobne raziskave nakazujejo številne koristne možnosti uporabe izrabljenega gobjega substrata. V projektni raziskavi se je IGS uporabljal kot organsko gnojilo na treh različnih lokacijah v ekološki, integrirani in biodinamični pridelavi paradižnika v rastlinjakih. V vsaki od pridelav se je v triplikatih testiralo gnojenje z SMS, standardno gnojenje (S), SMS+S in kontrola brez gnojenja. V sklopu magistrskega dela smo na obravnavanih lokacijah jemali vzorce zemlje v vseh letnih časih, v vzorcih pa smo analizirali aktivnost encimov N-acetil-glukozaminidaza (NAG), fosfataza (PHOS), beta-glukozidaza (β0GLU), arilsulfataza (ARS), ureaza (URE), arilamidaza (ARN), alkalna fosfataza (PAK) in kisla fosfataza (PAC) po standardu ISO 20130:2018- Kakovost tal - Merjenje encimske aktivnosti v vzorcih tal s kolorimetričnimi substrati na mikrotitrski plošči. Primerjali smo razlike v encimski aktivnosti med posameznimi letnimi časi ter med različnimi načini gnojenja. Rezultati so pokazali, da različni letni časi vplivajo na aktivnost encimov v tleh, medtem ko dodatek gobjega substrata kot gnojila ne povzroči nujno povišanja encimske aktivnosti.
Ključne besede: talni encimi, izrabljen gobji substrat, aktivnost, gnojenje, kmetijstvo
Objavljeno v DKUM: 03.09.2024; Ogledov: 52; Prenosov: 88
Celotno besedilo (3,58 MB)