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Rotaviral RNA found on various surfaces in a hospital laundry
Sabina Fijan, Andrej Steyer, Mateja Poljšak-Prijatelj, Avrelija Cencič, Sonja Šostar-Turk, Srečko Koren, 2008, original scientific article

Abstract: The aim of this investigative study was to determine the presence of rotaviral RNA at various control points (CP) of a hospital laundry. One of the possible sources of hospital infections is inappropriately laundered and disinfected hospital textiles. RT-PCR and nested PCR for gene amplification using specific primers following RNA isolation were used to determine the presence of rotaviral RNA on swabs. In addition, rotavirus suspensions were inoculated on marked surfaces as positive controls for different surfaces (cotton textiles, folding table and industrial dryer). Rotaviral RNA was found on various laundry surfaces: technical equipment, storage shelves, transport vehicles, personnel's hands, damp textiles, and folded laundry. Rotaviral RNA was also detected at all positive controls on tested surfaces after 24 h. Based on the results, it is very important to take into consideration the proper handling of textiles after washing as one of the precautions against hospital-acquired infections. This paper reports the presence of rotaviral RNA for the first time on surfaces in laundries and equipment, as well as textiles.
Keywords: laundry hygiene, rotaviruses, hospital-acquired disease prevention, sanitary measures, occupational health
Published in DKUM: 01.06.2012; Views: 2858; Downloads: 96
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Hygiene monitoring of textiles used in the food industry
Sabina Fijan, Avrelija Cencič, Sonja Šostar-Turk, 2006, original scientific article

Abstract: Protective clothing is required in the food-processing industry, to protect workers from contamination by bacteria, fungi, viruses, prions etc. contained in the secretions and raw meat of slaughtered animals, and to protect the meat from being contaminated by microorganisms carried by the workers. It is well-understood that textiles are a control point (CP), and must be appropriately cleaned and disinfected in order to prevent biocontamination. Although the laundering procedure itself is important for achieving disinfection, it is also essential to maintain an appropriate hygiene level in the laundry, in order to prevent recontamination of textiles by environmental viable microorganisms. In this study, a sanitary-microbiologicalanalysis was carried out in selected CPs in two laundries. Chemo-thermal washing efficiency was determined by evaluating the anti-bacterial effect against Enterococcus faecium and Staphylococcus aureus. The hygienic state of the laundries was determined by evaluating the number and type of microorganisms at selected CPs throughout the whole laundering procedure. The results indicated that the sanitary condition of both laundries did not reach the required levels and that several microbes were resistant to cleaning and disinfecting agents. It is obvious from the results that achievement of an appropriate hygiene level during laundering textiles from the food processing industry requires the implementation of appropriate corrective monitoring measures.
Keywords: laundry hygiene, textiles, food-processing industry, occupational health
Published in DKUM: 30.05.2012; Views: 2239; Downloads: 366
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