PRELIMINARNA RAZISKAVA VPLIVA RAZLIČNIH KEMIČNIH SPOJIN NA RAST IN RAZVOJ ŠKRLATNEGA AMERIŠKEGA SLAMNIKAEchinacea purpurea (L.)Moench V KONTROLIRANIH RAZMERAHIvana Vukovski
, 2010, undergraduate thesis
Abstract: Raziskava je potekala v rastlinjaku Fakultete za kmetijstvo in biosistemske vede Univerze
v Mariboru na Pivoli pri Hocah (SV Slovenija). Glavni cilj naloge je bil priti do osnovnih
informacij o vplivu nekaterih kemijskih substanc (jasmonska kislina, salicilna kislina,
EDDHA in PHOSAMCO) v razlicnih koncentracijah na rast in razvoj škrlatnega
ameriškega slamnika (Echinacea purpurea (L.) Moench) v kontroliranih razmerah. Mlade
sadike smo namocili za 24 ur v odgovarjajoce koncentracije kemikalij in jih nato posadili v
lonce. Fenotipska analiza je vkljucevala 12 lastnosti, opravljena pa je bila, ko so rastline
dosegle stadij petih listov. Raziskava je pokazala, da so bile najbolj ucinkovite jasmonska
kislina (pri koncentraciji 0,03 mM) in salicilna kislina (pri koncentracijah 0,06; 0,6 in 1,2 mM.
Keywords: Kljucne besede: Echinacea purpurea, škrlatni ameriški slamnik, jasmonska kislina, salicilna kislina, EDDHA, rast rastlin, razvoj rastlin, fenotipske lastnosti.
Published: 30.03.2010; Views: 2228; Downloads: 183
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Possible role of jasmonic acid in the regulation of floral induction, evocation and floral differentiation in Lemna minor L.Božidar Krajnčič
, Janja Kristl
, Ignac Janžekovič
, 2006, original scientific article
Abstract: Jasmonic acid (JA) is implicated in a wide variety of developmental and physiological processes in plants. Here, we studied the effects of JA and the combination of JA and ethylenediamine-dio-hydroxypheny-acetic acid (EDDHA) on flowering in Lemna minor in axenical cultures. JA (0.475-47.5 nmol l-1) enhanced floral induction in L. minor under long-day (LD) conditions. Under the same conditions, at the concentration of 237.5 nmol l-1, JA inhibited floral induction, and at the concentration of 475 nmol l-1 it prevented floral induction. Under LD conditions with LD preculture, a combination of EDDHA (20,500 nmol l-1) and JA (47.5 nmol l-1) had a synergistic effect on the promotion of floral induction. Floral induction was enhanced to the greatest extent in experiments with LD precultures. Microscopic examination of microphotographs of histological sections showed that JA and, to an even greater extent, JA+ EDDHA at optimal concentrations promote apical floral induction (evocation). Furthermore, and to an even greater extent JA in combination with EDDHA in an optimal concentration, also promote flower differentiation, especially the development of stamens, as is evident from themicrophotographs. The experimental results show that JA promotes floral induction in other species of Lemnaceae from various groups according to their photoperiodic response. The results support our hypothesis that, in addition to previosly ascribed functions, JA may regulate floral induction, evocation and floral differentiation. Our hypothesis is supported also by the results obtained by quantitative determination of endogenous JA levels in L. minor at three growth stages. The levels of endogenous JA decreased from 389 ng JA g-1 (fresh weight) of L. minor during the vegetative stage to 217 ng JA g-1 during the evocation stage, and to 37.5 ng JA g-1 during the flowering stage, which proves that JA is used for flowering.
Keywords: chelating agent EDDHA, flowering, jasmonic acid (JA), Lemna minor L., endogenous JA levels
Published: 10.07.2015; Views: 668; Downloads: 59
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The effects of jasmonic acid and ethylenediamine-di-o-hydroxyphenyl-acetic acid on floral induction and induction of turions in Spirodela polyrhiza (L.) SchleidenVirgilija Marinc Hrženjak
, Janja Kristl
, Božidar Krajnčič
, 2008, original scientific article
Abstract: We investigated the effects of jasmonic acid (JA) and ethylenediamine-di-o-hydroxyphenyl-acetic acid (EDDHA) on floral induction and induction of turions in the photoperiodically neutral clone Djelekovec of the species Spirodela polyrhiza in axenical cultures. JA (0.475-47.5 nmol.L-1) promoted floral induction under long-day (LD) and short day (SD) conditions, while it had no effect on turion induction. The inhibitory effect of JA on flowering and turion induction was observed at a JA concentration of 237.5 nmol.L-1 and 475 nmpl.L-1. Under the same conditions, flowering and induction of turions were enhanced by EDDHA (20.5 nmol..L-1(. The combination od EDDHA and JA had an additive effect on promotion of floral induction, which was promoted significantly in experiments with LD preculture. The results obtained by quantitative determination of endogenous JA levels in Spirodela polyrhiza at two growth stages support our previous findings that JA may regulate floral induction. The levels of endogenous JA decreased from 226±12 ng·g–1 fresh weight during the vegetative stage in LD to 38.1±3.5 ng·g–1 in the flowering plants. In SD the levels of endogenous JA decreased from 62±9ng·g–1 fresh weight during vegetative stage to 29.2±3.1 ng·g–1 in the flowering plants.
Keywords: Spirodela polyrhiza (L.) Schleiden, chelating agent EDDHA, flowering, induction of turions, jasmonic acid
Published: 28.03.2017; Views: 445; Downloads: 50
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