1. Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell linesMaja Šikić Pogačar, Tomaž Langerholc, Dušanka Mičetić-Turk, Sonja Smole Možina, Anja Klančnik, 2020, original scientific article Abstract: Background : Campylobacter spp. are a major cause of bacterial food-borne diarrhoeal disease. This mainly arises through contamination of meat products during processing. For infection, Campylobacter spp. must adhere to epithelial cells of the mucus layer, survive conditions of the gastrointestinal tract, and colonise the intestine of the host. Addition of probiotic bacteria might promote competitive a dhesion to epithelial cells, consequently reducing Campylobacter jejuni colonisation. Effect of Lactobacillus spp. (PCS20, PCS22, PCS25, LGG, PCK9) on C. jejuni adhesion, invasion and translocation in pig (PSI cl.1) and chicken (B1OXI) small-intestine cell lines, as well as pig enterocytes (CLAB) was investigated.
Results : Overall, in competitive adhesion assays with PSI cl.1 and CLAB cell monolayers, the addition of Lactobacillus spp. reduced C. jejuni adherence to the cell surface, and negatively affected the C. jejuni invasion. Interestingly, Lactobacillus spp. significantly impaired C. jejuni adhesion in three-dimensional functional PSI cl.1 and B1OXI cell models. Also, C. jejuni did not translocate across PSI cl.1 and B1OXI cell monolayers when co-incubated with probiotics. Among selected probiotics, Lactobacillus rhamnosus LGG was the strain that reduced adhesion efficacy of C. jejuni most significantly under co-culture conditions.
Conclusion : The addition of Lactobacillus spp. to feed additives in livestock nutrition might be an effective novel strategy that targets Campylobacter adhesion to epithelial cells, and thus prevents colonisation, reduces the transmission, and finally lowers the incidence of human campylobacteriosis.
Keywords: Campylobacter jejuni, Lactobacillus spp., chicken and pig cell line, adhesion, invasion, translocation
Published in DKUM: 06.02.2025; Views: 0; Downloads: 2
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2. Economic viability of alternative bedding material in broiler chicken farmingJernej Prišenk, Maksimiljan Brus, 2022, original scientific article Abstract: The aim of this paper is to assess the economic viability of two different bedding materials used in broiler chicken farming processes. The materials considered are wood shavings and slice-dedusted straw. The slice-dedusted straw is considered an alternative type of bedding material produced by a company from Slovenia. While the technological and economic assessment of this type of bedding material has already been researched in the case of horse breeding, it is something new in the case of broiler chicken farming. Data collection is structured from two trials. Trial one (T1) is also known as daily observations, and trial two (T2) involves obtaining input data at the end of the fattening period. During T1, daily observations are focused on collecting data from technical characteristics, and in T2, the percentage of death proportions and average increments are observed, calculated, and considered as economic input data. The cost calculation model is used for the calculation of several different technical-economic indicators, which denote the influence of different bedding materials no economic production viability. Favorable economic results were found for slice-dedusted straw, which shows that this kind of alternative bedding material could be the better option.
Keywords: bedding materials, feasibility analysis, broiler chicken, cost calculation model
Published in DKUM: 02.07.2024; Views: 111; Downloads: 19
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3. Composite materials based on waste chicken feather fibers for oil-spill managementSimona Strnad, Andraž Jug, Zdenka Peršin Fratnik, 2024, original scientific article Abstract: Oil spills remain one of the greatest man-made ecological threats, despite numerous advanced cleanup approaches. They still pose a major challenge in the search for materials and technologies that work as efficiently and sustainably as possible. Promising natural materials include poultry feathers, which are produced in large quantities every day as a byproduct of the meat industry. In this study, the influence of different forms of absorbents (loose feathers, pillows, and sheets) based on chicken feathers and the addition of an inorganic absorbent, sepiolite, on their absorption capacity was investigated. The chemical and physical surface properties, like morphology, chemical composition, zeta potential, surface free energies and oil absorption capacities were analyzed. The Gibbs free energy of immersion wetting with oil and the work of adhesion of the adsorbents, calculated based on contact angle measurements, were confirmed by the tests of adsorption capacities according to the ASTM 726–12 standard. The results showed that pure loose feathers have the highest oil adsorption capacity, while feather pillows have only half, and composite sheets have only a quarter of this capacity. The addition of inorganic adsorbent sepiolite did not increase the absorption capacity of the composites.
Keywords: chicken feather fibers, composites, sepiolite, surface properties, oil adsorption, oil-spill management
Published in DKUM: 10.05.2024; Views: 183; Downloads: 23
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4. The function of language in characterization : dialectal speech in the animated film Chicken LittleTina Cupar, Alenka Valh Lopert, 2014, original scientific article Abstract: The article discusses the use of language varieties by the main character in the animated film Chicken Little in English and Slovene. Both versions of the film are dubbed by professional actors and are aimed at a young target audience, children. The main intention of the article is to analyze the characteristics of Chicken Little’s speech in both languages, to compare the differences in the use of language varieties, and to evaluate the consequences of shifts in language use on the character and the story in the target language. The analysis is based on a transcript of the speech and enables comparison on four different levels: phonetics, morphology, syntax and vocabulary. The main focus is on the analysis of speech in the target language: Maribor regional colloquial language, with influence from the dialectal speech of Ruše. The main conditions influencing the use of certain language varieties are taken into consideration: the characteristics of the dubbing process, specifics of the target audience, and prevailing norms related to the use of language on television.
Keywords: Slovene language, dialectal speech, varieties of language, animated films, Chicken Little, dubbing, children’s literature
Published in DKUM: 16.05.2017; Views: 1530; Downloads: 180
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5. Comparison of George Orwell’s novel Animal Farm and animation film Chicken RunTadeja Premrov, 2013, undergraduate thesis Abstract: The aim of this thesis was to compare George Orwell’s novel Animal Farm and the animated film Chicken Run. The theme in both works is a rebellion, so I compared its reasons and consequences. I compared farms, farm owners, revolt managers (Napoleon and Ginger) and other characters, and lastly analyzed and compared both works as allegories. I came to the conclusion that both revolts were stimulated by masters’ evil characteristics and lack of care and kindness for animals. However, not both revolts ended with freedom and prosperity for animals. I highlighted the reasons for this discrepancy. The analysis of allegories showed that both, the novella and the film, use animal characters to construct an idea of how people were oppressed in the history, each highlighting a different part of European history.
Keywords: Animal Farm, Chicken Run, a revolt, a rebellion, comparison, allegory.
Published in DKUM: 25.09.2013; Views: 3489; Downloads: 260
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