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Optimizacija encimatske hidrolize tekstilnih odpadkov iz mešanice poliestra in celuloze
Leon Škrabl, 2024, undergraduate thesis

Abstract: Tekstilni odpadki oz. odpadni material predstavlja velik in rastoč problem v današnji potrošniški družbi, zato smo se v tej diplomski nalogi osredotočili na encimatsko hidrolizo celuloze v materialu, ki vsebuje dve tretjini polietilen tereftalata in eno tretjino celuloze. Preizkusili smo različne pogoje, torej kakšen je vpliv različnih temperatur, koncentracij encimske mešanice (Cellic® CTec2) in dveh različnih pufrskih raztopin. V prvem delu poskusa smo testirali, ali 40 % povečanje količine encimske mešanice pomeni hitrejšo razgradnjo celuloze oziroma višjo koncentracijo glukoze v vzorcih. Ugotovili smo, da večja količina encimske mešanice pomeni malo boljše rezultate, ampak je ta razlika minimalna in nezadostna, da bi bila uporaba večje količine ecimske mešanice smotrna. Prav tako smo preverili, kako reakcija poteka v prisotnosti 0,1 M pufrske raztopine (NH4)2SO4 in 0,1 M pufrske raztopine NH4Cl. Rezultati so pokazali, da je precej več glukoze nastalo v pufrski raztopini (NH4)2SO4 in tudi poraba NaOH je bila veliko manjša. V drugem delu poskusa pa nas je zanimalo, kakšen vpliv na reakcijo in encimsko mešanico imajo tri različne temperature, in sicer 50, 55 in 60 °C. Iz podatkov je razvidno, da je najoptimalnejša temperatura 50 °C, saj je v tem reaktorju nastalo največ glukoze.
Keywords: celuloza, Cellic® CTec2, encimatska hidroliza, (NH4)2SO4, NH4Cl
Published in DKUM: 10.09.2024; Views: 24; Downloads: 7
Full text (3,71 MB)

Optimization of conditions for enzymatic decomposition of fibrous cotton textile waste : diplomsko delo univerzitetnega študijskega programa I. stopnje
Stela Tashkova, 2023, undergraduate thesis

Abstract: The main goal of this thesis was to optimize the conditions under which a 100 % cellulose material enzymatically decomposes. Waste textile and waste cellulose represent a large source of carbon that can be used in the production of value–added chemicals. The main goal of this diploma thesis was to test different conditions for the enzymatic decomposition of cellulose waste such as temperature range, enzymes concentration and different buffer composition. We started with the optimization of the temperature and tested how the reaction proceeds at three different temperatures (50, 55 and 60 °C). Here, the main goal was to check how the enzymatic mixture we used acts at these three different environments, thus how much will the material decompose. The results showed that the optimal temperature for the decomposition of cellulose is 50 °C. The second part of the diploma thesis evaluates if a higher concentration of enzymes would get better results regarding cellulose waste decomposition. The results here showed that an increased enzyme concentration does not improve the efficiency of the reaction under the tested conditions. Additionally, two different buffers 0.1 M NH4Cl and 0.1 M (NH4)2SO4 were tested as environments for the enzymatic degradation of cellulose waste. We got a higher glucose concentration in the reaction with (NH4)2SO4, as well as less variability of the pH, which led to a lower NaOH consumtion.
Keywords: enzymatic cellulose decomposition, optimization, temperature effect, NH4Cl, (NH4)2SO4, Cellic® CTec2
Published in DKUM: 13.09.2023; Views: 373; Downloads: 20
Full text (13,48 MB)

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