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Variations in leaf total protein, phenolic and thiol contents amongst old varieties of mulberry from the Gorizia region
Tina Ugulin, Tamas Bakonyi, Rebeka Lucijana Berčič, Andreja Urbanek Krajnc, 2015, original scientific article

Abstract: Preserving the plant genetic resources of genus Morus is insuffcient but undoubtedly vital for conservation of the world’s germplasm for our successors. This research was focused on old mulberry varieties from the Gorizia region in Slovenia which were assessed for their contents on crucial metabolites (proteins, phenolics and thiols) in leaves regarding their antioxidant and nutraceutical potentials. Total proteins were measured spectrophotometrically by following the procedure of Bradford, the total phenolic contents were determined using the Folin-Ciocalteu method and thiols were established with monobromobimane fluorescent dye. The presented metabolite screening showed that some of the evaluated genotypes had higher concentrations of glutathione and were superior in contents of proteins and phenolics when compared to the results of other authors and could be propagated as highly recommendable feed for silkworms, and other animals.
Keywords: feeds, glutathione, Morus, phenols, proteins
Published in DKUM: 14.11.2017; Views: 1309; Downloads: 473
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Viral infections in queen bees (Apis mellifera carnica) from rearing apiaries
Aleš Gregorc, Tamás Bakonyi, 2012, original scientific article

Abstract: Viral infection could have an impact on the success of queen rearing and a potential effect on reduced queen quality. Newly mated honey bee (Apis mellifera carnica) queens were collected from mating nuclei in queen rearing operations in Slovenia. Altogether, 81 queens were sampled from 27 rearing apiaries in 2006 and 72 queens from 24 apiaries in 2008. Queens were analysed for the presence of four viruses: acute bee paralysis virus (ABPV), black queen cell virus (BQCV), sacbrood virus (SBV) and deformed wing virus (DWV) by using reverse transcription polymerase chain reaction (RT-PCR). In 2006, 12%, 9% and 1% prevalence was found for ABPV, DWV and SBV, respectively; BQCV was not detected. Two years later, DWV, BQCV, SBV and ABPV were detected in 58%, 24%, 11% and 10% bee queens, respectively. In 2006, fourteen out of twenty-seven apaiaries were virus free, whereas in 2008 only three out of twenty-four apiaries were virus free. This is the first evidence of virus infection occurring in newly mated queens from mating nuclei in rearing apiaries. The possible impacts of queen rearing technology and epidemiological influences on virus infection are discussed in this study.
Keywords: queen bees, black queen cell virus, acute bee paralysis virus, sacbrook virus, deforming wing virus
Published in DKUM: 04.08.2017; Views: 998; Downloads: 354
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