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Abstract: issue engineering faces the challenge of achieving effective vascularization within tissue constructs for sustained viability andoptimal function. The success of tissue-engineered constructs depends on selecting an optimal angiogenesis-stimulating ECMsubstitute material. This study compares four substrates made from three different biomacromolecules—fibrin, fibronectin,non-crosslinked, and crosslinked gelatin, and their effect on endothelial cells. Acknowledging the diverse range of endothelialcells that play a role in (micro)vascularization, human endothelial primary cells, human umbilical vein endothelial cells, andhuman microvascular endothelial cells are subjected to these materials for evaluation. Biocompatibility is assessed by measuringcell viability (Live/Dead assay), metabolic activity (alamarBlue assay), morphology (actin staining), phenotype expression(immunocytochemistry), and the production of von Willebrand factor, which promotes angiogenesis by promoting cell adhesionand migration. The results show that the use of biomaterials as culturing substrates significantly impacts the viability andmorphology of the cells. While the expression of angiogenic markers is shown to rely more on the cell lineage, the use of differentsubstrates has an impact on the expression timeline. Thus, combining cells and biomaterials in a favorable manner can be usedas a powerful tool for controlled vascularization in vitro, which requires the systematic assembly of different stimuli.
Keywords: biomacromolecules, endothelial cell types, materials, microvascularization, vascularization
Published in DKUM: 22.07.2025; Views: 0; Downloads: 17
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Abstract: Mesenchymal stem cells (MSCs) represent the basis of novel clinical concepts in cellular therapy and tissue regeneration. Therefore, the isolation of MSCs from various tissues has become an important endeavour for stem cell biobanking and the development of regenerative therapies. Paravertebral adipose tissue is readily exposed during spinal procedures in children and could be a viable source of stem cells for therapeutic applications. Here, we describe the first case of MSCs isolated from paravertebral adipose tissue (PV-ADMSCs), obtained during a routine spinal surgery on a child. Using quantitative real-time PCR and flow cytometry, we show that PV-ADMSCs have different levels of stem marker expression compared to the MSCs from other sources while having the highest proliferation rate. Furthermore, we evaluate the multipotency of PV-ADMSCs by the three-lineage (adipogenic, osteogenic and chondrogenic) differentiation and compare it to the multipotency of MSCs from other sources. It was found that the PV-ADMSCs have a strong osteogenic potential in particular. Taken together, our data indicate that PV-ADMSCs meet the criteria for successful cell therapy, defined by the International Society for Cellular Therapy (ISCT), and thus, could provide a source of MSCs that is relatively easy to isolate and expand in culture. Due to their strong osteogenic potential, these cells provide a promising basis, especially for orthopaedic applications.
Keywords: mesenchymal stem cells, paediatric surgery, paravertebral adipose tissue, regenerative medicine, stem marker expression, osteogenic potential, differentiation
Published in DKUM: 16.12.2024; Views: 0; Downloads: 13
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Abstract: The development of novel polymer-based materials opens up possibilities for several novel applications, such as advanced wound dressings, bioinks for 3D biofabrication, drug delivery systems, etc. The aim of this study was to evaluate the viability of vascular and intestinal epithelial cells on different polymers as a selection procedure for more advanced cell-polymer applications. In addition, possible correlations between increased cell viability and material properties were investigated. Twelve polymers were selected, and thin films were prepared by dissolution and spin coating on silicon wafers. The prepared thin films were structurally characterized by Fourier transform infrared spectroscopy, atomic force microscopy, and goniometry. Their biocompatibility was determined using two epithelial cell lines (human umbilical vein endothelial cells and human intestinal epithelial cells), assessing the metabolic activity, cell density, and morphology. The tested cell lines showed different preferences regarding the culture substrate. No clear correlation was found between viability and individual substrate characteristics, suggesting that complex synergistic effects may play an important role in substrate design. These results show that a systematic approach is required to compare the biocompatibility of simple cell culture substrates as well as more complex applications (e.g., bioinks).
Keywords: HUIEC, HUVEC, morphology, polymers, thin films, viability
Published in DKUM: 18.10.2024; Views: 0; Downloads: 9
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Abstract: The ethical constraints and shortcomings of animal models, combined with the demand to study disease pathogenesis under controlled conditions, are giving rise to a new field at the interface of tissue engineering and pathophysiology, which focuses on the development of in vitro models of disease. In vitro models are defined as synthetic experimental systems that contain living human cells and mimic tissue- and organ-level physiology in vitro by taking advantage of recent advances in tissue engineering and microfabrication. This review provides an overview of in vitro models and focuses specifically on in vitro disease models of the endocrine pancreas and diabetes. First, we briefly review the anatomy, physiology, and pathophysiology of the human pancreas, with an emphasis on islets of Langerhans and beta cell dysfunction. We then discuss different types of in vitro models and fundamental elements that should be considered when developing an in vitro disease model. Finally, we review the current state and breakthroughs in the field of pancreatic in vitro models and conclude with some challenges that need to be addressed in the future development of in vitro models.
Keywords: in vitro disease models, pancreas, islet of Langerhans, 3D cell culture, scaffolds, acute tissue slices
Published in DKUM: 01.10.2024; Views: 0; Downloads: 426
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Abstract: Uvod: Brezšivna skrb je na posameznika usmerjen proces, katere glavni namen je preverjanje skladnosti predpisane farmakološke terapije s ciljem zmanjšanja napak, ki se pojavljajo v povezavi s farmakološko terapijo. Gre za proces, ki zahteva multidisciplinarno sodelovanje strokovnjakov na različnih ravneh zdravstvenega varstva. Metode: V teoretičnem delu magistrskega dela smo uporabili deskriptivno metodo analize in sinteze virov, pridobljenih s pomočjo domače in tuje znanstvene ter strokovne literature. V empiričnem delu magistrskega dela smo izvedli intervjuje s kliničnimi farmacevti v izbrani bolnišnični instituciji, kot instrument raziskovanja pa smo uporabili pol strukturiran intervju. Rezultati: Na podlagi analize in sinteze virov ter izvedenih intervjujev smo ugotovili, da brezšivna skrb pomembno vpliva na varnost in kakovost obravnave hospitaliziranih pacientov s pridruženimi kroničnimi nenalezljivimi boleznimi in da imajo intervjuvanci dobro percepcijo glede implementacije brezšivne skrbi v prakso. Razprava in zaključek: Brezšivna skrb pomembno vpliva na zmanjševanje napak, ki se pojavljajo v povezavi s farmakološko terapijo v praksi in so pogostejše, kot si predstavljamo. Posledično lahko izpostavimo prednosti tako za posameznika kot javno-zdravstveni sistem.
Keywords: Brezšivna skrb, pacient, kronične nenalezljive bolezni, usklajevanje zdravljenja z zdravili, klinični farmacevt.
Published in DKUM: 22.04.2024; Views: 278; Downloads: 73
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