| | SLO | ENG | Cookies and privacy

Bigger font | Smaller font

Search the digital library catalog Help

Query: search in
search in
search in
search in
* old and bologna study programme

Options:
  Reset


1 - 10 / 12
First pagePrevious page12Next pageLast page
1.
Nanodelci hitozana kot potencialni protimikrobni premaz
Zdenka Peršin Fratnik, Uroš Maver, Metod Kolar, Olivera Šauperl, Lidija Fras Zemljič, Boštjan Vihar, 2024, original scientific article

Abstract: Namen študije je bil ugotoviti protimikrobno učinkovitost hitozanskih nanodelcev v primerjavi s hitozansko raztopino in sled temu njihovo učinkovito rabo na medicinskih tekstilnih materialih.
Keywords: chitosan, nanoparticles, antimicrobial activity, coatings, medical textile materials
Published in DKUM: 07.01.2025; Views: 0; Downloads: 17
.pdf Full text (903,83 KB)
This document has many files! More...
This document is also a collection of 1 document!

2.
Kratek pregled platform za ADME testiranje
Tina Maver, Boštjan Vihar, Uroš Maver, 2024, original scientific article

Abstract: V zadnjem času je bil dosežen pomemben napredek pri razvoju ADME (absorpcija, distribucija, metabolizem, ekskrecija) modelov, vendar izziv ostaja vzpostaviti platforme, ki bi zmanjšale testiranje na živalih in stroške raziskav. Naraščajoča pomembnost farmakokinetičnih interakcij poudarja potrebo po zanesljivih in ponovljivih ADME modelih, ki so vse bolj ključni za razvoj zdravil in zagotavljanje varnosti z željo po preprečevanju resnih kliničnih zapletov in hospitalizacije.
Keywords: ADME, drug interaction studies, in vitro models, pharmacokinetics, multi-organ models
Published in DKUM: 07.01.2025; Views: 0; Downloads: 17
.pdf Full text (405,16 KB)
This document has many files! More...
This document is also a collection of 1 document!

3.
Mesenchymal stem cells isolated from paediatric paravertebral adipose tissue show strong osteogenic potential
Jan Rožanc, Lidija Gradišnik, Tomaž Velnar, Minja Gregorič, Marko Milojević, Boštjan Vihar, Boris Gole, Uroš Maver, 2022, original scientific article

Abstract: Mesenchymal stem cells (MSCs) represent the basis of novel clinical concepts in cellular therapy and tissue regeneration. Therefore, the isolation of MSCs from various tissues has become an important endeavour for stem cell biobanking and the development of regenerative therapies. Paravertebral adipose tissue is readily exposed during spinal procedures in children and could be a viable source of stem cells for therapeutic applications. Here, we describe the first case of MSCs isolated from paravertebral adipose tissue (PV-ADMSCs), obtained during a routine spinal surgery on a child. Using quantitative real-time PCR and flow cytometry, we show that PV-ADMSCs have different levels of stem marker expression compared to the MSCs from other sources while having the highest proliferation rate. Furthermore, we evaluate the multipotency of PV-ADMSCs by the three-lineage (adipogenic, osteogenic and chondrogenic) differentiation and compare it to the multipotency of MSCs from other sources. It was found that the PV-ADMSCs have a strong osteogenic potential in particular. Taken together, our data indicate that PV-ADMSCs meet the criteria for successful cell therapy, defined by the International Society for Cellular Therapy (ISCT), and thus, could provide a source of MSCs that is relatively easy to isolate and expand in culture. Due to their strong osteogenic potential, these cells provide a promising basis, especially for orthopaedic applications.
Keywords: mesenchymal stem cells, paediatric surgery, paravertebral adipose tissue, regenerative medicine, stem marker expression, osteogenic potential, differentiation
Published in DKUM: 16.12.2024; Views: 0; Downloads: 5
.pdf Full text (3,70 MB)
This document has many files! More...

4.
Investigating the viability of epithelial cells on polymer based thin-films
Boštjan Vihar, Jan Rožanc, Boštjan Krajnc, Lidija Gradišnik, Marko Milojević, Laura Činč Ćurić, Uroš Maver, 2021, original scientific article

Abstract: The development of novel polymer-based materials opens up possibilities for several novel applications, such as advanced wound dressings, bioinks for 3D biofabrication, drug delivery systems, etc. The aim of this study was to evaluate the viability of vascular and intestinal epithelial cells on different polymers as a selection procedure for more advanced cell-polymer applications. In addition, possible correlations between increased cell viability and material properties were investigated. Twelve polymers were selected, and thin films were prepared by dissolution and spin coating on silicon wafers. The prepared thin films were structurally characterized by Fourier transform infrared spectroscopy, atomic force microscopy, and goniometry. Their biocompatibility was determined using two epithelial cell lines (human umbilical vein endothelial cells and human intestinal epithelial cells), assessing the metabolic activity, cell density, and morphology. The tested cell lines showed different preferences regarding the culture substrate. No clear correlation was found between viability and individual substrate characteristics, suggesting that complex synergistic effects may play an important role in substrate design. These results show that a systematic approach is required to compare the biocompatibility of simple cell culture substrates as well as more complex applications (e.g., bioinks).
Keywords: HUIEC, HUVEC, morphology, polymers, thin films, viability
Published in DKUM: 18.10.2024; Views: 0; Downloads: 1
.pdf Full text (20,37 MB)
This document has many files! More...

5.
Microvascular tissue engineering : a review
Jernej Vajda, Marko Milojević, Uroš Maver, Boštjan Vihar, 2021, review article

Abstract: Tissue engineering and regenerative medicine have come a long way in recent decades, but the lack of functioning vasculature is still a major obstacle preventing the development of thicker, physiologically relevant tissue constructs. A large part of this obstacle lies in the development of the vessels on a microscale%the microvasculature%that are crucial for oxygen and nutrient delivery. In this review, we present the state of the art in the field of microvascular tissue engineering and demonstrate the challenges for future research in various sections of the field. Finally, we illustrate the potential strategies for addressing some of those challenges.
Keywords: regenerative medicine, tissue engineering, vascularization, biomaterials, microvascularization, coculture, gradients
Published in DKUM: 18.10.2024; Views: 0; Downloads: 2
.pdf Full text (6,81 MB)
This document has many files! More...

6.
In vitro disease models of the endocrine pancreas
Marko Milojević, Jan Rožanc, Jernej Vajda, Laura Činč Ćurić, Eva Paradiž, Andraž Stožer, Uroš Maver, Boštjan Vihar, 2021, review article

Abstract: The ethical constraints and shortcomings of animal models, combined with the demand to study disease pathogenesis under controlled conditions, are giving rise to a new field at the interface of tissue engineering and pathophysiology, which focuses on the development of in vitro models of disease. In vitro models are defined as synthetic experimental systems that contain living human cells and mimic tissue- and organ-level physiology in vitro by taking advantage of recent advances in tissue engineering and microfabrication. This review provides an overview of in vitro models and focuses specifically on in vitro disease models of the endocrine pancreas and diabetes. First, we briefly review the anatomy, physiology, and pathophysiology of the human pancreas, with an emphasis on islets of Langerhans and beta cell dysfunction. We then discuss different types of in vitro models and fundamental elements that should be considered when developing an in vitro disease model. Finally, we review the current state and breakthroughs in the field of pancreatic in vitro models and conclude with some challenges that need to be addressed in the future development of in vitro models.
Keywords: in vitro disease models, pancreas, islet of Langerhans, 3D cell culture, scaffolds, acute tissue slices
Published in DKUM: 01.10.2024; Views: 0; Downloads: 7
.pdf Full text (4,42 MB)
This document has many files! More...

7.
Novel magnetic iron oxide-dextran sulphate nanocomposites as potential anticoagulants: Investigating interactions with blood components and assessing cytotoxicity
Olivija Plohl, Lidija Fras Zemljič, Boštjan Vihar, Alenka Vesel, Sašo Gyergyek, Uroš Maver, Irena Ban, Matej Bračič, 2024, original scientific article

Abstract: Examining the critical role of anticoagulants in medical practice, particularly their central function in preventing abnormal blood clotting, is of the utmost importance. However, the study of interactions between blood proteins and alternative anticoagulant nano-surfaces is still understood poorly. In this study, novel approach involving direct functionalisation of magnetic iron oxide nanoparticles (MNPs) as carriers with sulphated dextran (s-dext) is presented, with the aim of evaluating the potential of magnetically-responsive MNPs@s-dext as anticoagulants. The physicochemical characterisation of the synthesised MNPs@s-dext includes crystal structure analysis, morphology study, surface and electrokinetic properties, thermogravimetric analysis and magnetic properties` evaluation, which confirms the successful preparation of the nanocomposite with sulfonate groups. The anticoagulant potential of MNPs@s-dext was investigated using a standardised activated partial thromboplastin time (APTT) test and a modified APTT test with a quartz crystal microbalance with dissipation (QCM-D) which confirmed the anticoagulant effect. Time-resolved solid-liquid interactions between the MNPs@s-dext and model blood proteins bovine serum albumin and fibrinogen were also investigated, to gain insight into their hemocompatibility, and revealed protein-repellence of MNPs@s-dext against blood proteins. The study also addressed comprehensive cytotoxicity studies of prepared nanocomposites, and provided valuable insights into potential applicability of MNPs@s-dext as a promising magnetic anticoagulant in biomedical contexts.
Keywords: dextran sulphate, magnetic nanoparticles, blood protein interactions, clot formation, anticoagulants, cytotoxicity studies
Published in DKUM: 25.07.2024; Views: 107; Downloads: 20
.pdf Full text (892,02 KB)
This document has many files! More...

8.
9.
10.
Search done in 0.29 sec.
Back to top
Logos of partners University of Maribor University of Ljubljana University of Primorska University of Nova Gorica