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1.
Diversity and content of carotenoids and other pigments in the transition from the green to the red stage of Haematococcus pluvialis microalgae identified by HPLC-DAD and LC-QTOF-MS
Jaša Veno Grujić, Biljana Todorović, Roman Kranvogl, Terezija Ciringer, Jana Ambrožič-Dolinšek, 2022, original scientific article

Abstract: H. pluvialis is a unicellular freshwater alga containing many bioactive compounds, especially carotenoids, which are the strongest antioxidants among the pigments. This study evaluates the composition and content of carotenoids and other pigments in both stages of algae life cycle, especially in the green vegetative stage, less studied in comparison to the red stage. To determine the composition and content of carotenoids, a combination of HPLC-DAD and LC-QTOF-MS was used. The content of carotenoids in the green vegetative stage was significantly lower than in the red vegetative stage. In the green vegetative stage, 16 different carotenoids and other pigments were identified. Among the total 8.86 mg g−1 DW of pigments, 5.24 mg g−1 DW or 59% of them were chlorophyll a with its derivatives, and 3.62 mg g−1 DW or 41% of them were free carotenoids. After the transition from the green to the red stage, the carotenoid composition was replaced by secondary carotenoids, astaxanthin and its esters, which predominated in the whole carotenoid composition. In addition to free astaxanthin, 12 astaxanthin monoesters, 6 diesters and 13 other carotenoids were determined. The majority of 37.86 mg g−1 DW pigments were monoesters. They represented 82% of all pigments, and their content was about 5 times higher than both, diesters (5.91 mg g−1 DW or 12% of all) and free carotenoids (2.4 mg g−1 DW or 6% of all). The results of the study contribute to the data on the overall pigment composition and content of H. pluvialis algae and provide the basis for further improvement of cultivation of the H. pluvialis algae.
Keywords: antioxidants, astaxanthin, chlorophylls, bioactive compounds, algae, Haematococcus, life cycle, pigment composition, secondary carotenoids
Published in DKUM: 21.05.2024; Views: 172; Downloads: 8
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2.
The potential of berula erecta in vitro for As bioaccumulation and phytoremediation of water environments
Jana Ambrožič-Dolinšek, Anja Podgrajšek, Nik Šabeder, Zdenka Mazej, Andreja Urbanek Krajnc, Biljana Todorović, Terezija Ciringer, 2023, original scientific article

Abstract: A potential plant species suitable for As bioaccumulation and phytoremediation of water environments could be the macrophyte Berula erecta. The objective of the present study was to investigate the effect of arsenate (As(V), C2H6AsNaO2∙3 H2O), predominant in freshwater systems, on the growth, development and low molecular weight thiols of Berula erecta under controlled tissue culture conditions in vitro. Uptake of total arsenate increased with increasing arsenate treatments, at a higher percentage in the roots than in the aboveground parts of the plants. Lower concentrations of As(V) (0.1, 1, 10 mg L−1) had a positive effect on growth, dry weight, length of roots and shoots and number of buds. High concentrations of arsenate (50 and 100 mg As(V) L−1) significantly inhibited all growth parameters and decreased the photochemical efficiency of PSII. Evaluation of thiols revealed the critical As level (146 µg g−1 DW; 50 mg As(V) L−1 treatment) above which the As concentration can be toxic.
Keywords: arsenic, arsenate, uptake, accumulation, thiols, apiaceae, lesser water-parsnip
Published in DKUM: 02.08.2023; Views: 446; Downloads: 85
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UPORABA PCA METODE PRI VREDNOTENJU VPLIVA POLOŽAJA PLODOV V KROŠNJI NA LASTNOSTI PRIDELKA PLODOV RAZLIČNIH SORT JABLAN (Malus domestica B.)
Biljana Todorović, 2011, master's thesis

Abstract: Uporaba PCA metode pri vrednotenju vpliva položaja plodov v krošnji na lastnosti pridelka plodov različnih sort jablan (Malus domestica B.) UDK: 634.11:519.87: 631.524. 7(043)=863 V Sadjarskem centru Maribor (Gačnik) in na posestvu UKC Pohorski dvor je bila v letu 2009 izvedena raziskava z namenom ovrednotiti vpliv položaja ploda v krošnji na nekatere parametre zrelosti in kakovosti pridelka jablan sort ′Gala′, ′Elstar′, ′Jonagold′, ′Braeburn′ in ′Fuji′. Sončni položaj je bil definiran kot položaj na obodu krošnje, polsenčni položaj med obodom krošnje in deblom in senčni položaj tik ob deblu. Za obdelavo podatkov sta bili uporabljeni PCA analiza in ANOVA. Skupna PCA analiza podatkov vseh petih sort jablan je pokazala močan vpliv položaja ploda v krošnji na testirane parametre zrelosti in kakovosti plodov (pojasnjeno 61 % variance). Pri vseh petih sortah je imel položaj ploda v krošnji signifikanten vpliv na intenzivnost obarvanosti povrhnjice, vsebnost topne suhe snovi in povprečno maso plodov, tako da so bili plodovi na sončnem položaju bolje obarvani, slajši in so dosegli večjo povprečno maso. Pri sortah 'Elstar' in 'Braeburn' so bili na sončnem položaju plodovi tudi trši in zrelejši. Pri sorti 'Fuji' je bila zrelost plodov najmanj odvisna od njihovega položaja v krošnji.
Keywords: jablana, PCA metoda, položaj plodov, kakovost, zrelost
Published in DKUM: 29.06.2011; Views: 2989; Downloads: 246
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