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11.
Zagotavljanje varnosti gensko spremenjenih živil v povezavi s prehransko alergijo
Gregor Ornik, 2011, master's thesis

Abstract: Varnost hrane je lahko širok pojem, zato je bil namen naloge spoznati varnost novo izraženih proteinov v GSO živilih glede na njihovo povezavo s prehranskimi alergijami. Analiza poročil Evropske agencije za varno hrano in druga strokovna literatura sta pokazali, da na tržišču prisotna GSO živila izpolnjujejo kriterije varne hrane in jim ni mogoče pripisati lastnosti povzročiteljev alergičnih reakcij. Najprej smo ugotavljali, kateri GSO se sploh uporabljajo v prehranske namene in kateri so tisti novi proteini, ki so lahko potencialni alergeni za človeka. Glavno vodilo, zraven zakonodajnega področja varnosti GSO živil, je bila ocena alergenosti novo izraženih proteinov, ki jo izdaja omenjena agencija. Smernice ocene alergenosti so nam dale osnovo za meta analitično analizo novo izraženih proteinov CP4 ESPSP in PAT ter njihovo povezavo s prehranskimi alergijami. Analiza je bila narejena na podlagi iskanja zaporedij in strukturnih podrobnostih, IgE testiranj, odpornosti na pepsin, kartiranja specifičnih serumov in uporabe živalskih modelov. Na podlagi strokovne literature smo ugotovili, da proteina na teh testiranjih nista pokazala lastnosti, ki jih imajo znani alergeni. Tako smo predpostavili, da transgena verjetno ne povzročata alergičnih reakcij in sta za potrošnika najverjetneje varna.
Keywords: prehranska alergija, GSO, novo izražen protein, ocena alergenosti
Published: 24.11.2011; Views: 2064; Downloads: 222
.pdf Full text (794,65 KB)

12.
Biološka aktivnost ekstrakta jagod (Fragaria x ananassa L. Duch.)
Sara Krajnc, 2012, undergraduate thesis

Abstract: Rak je zelo zapletena bolezen. Ne moremo je opisati tako natančno kot na primer kako nalezljivo bolezen, pri kateri poznamo povzročitelja, način okužbe, bolezenske spremembe in način obrambe mehanizma, pa tudi uspešno zdravljenje in zaščito. Splošno znano je, da lahko uživanje zadostne količine sadja veliko pripomore k zdravemu načinu življenja. Na kar pa je vredno opozoriti, pa je to, da lahko veliko sadja pridelamo doma na vrtu brez tretiranja s fitofarmacevtskimi sredstvi, ki so danes nepogrešljiv kemični produkt za zaviranje škodljivcev. Veliko raziskav je bilo narejenih z jagodami, ker naj bi bile snovi v jagodah uspešen zaviralec rasti rakavih celic. Namen naše diplomske naloge je bil, da na podlagi raziskav oz. poizkusov dokažemo, da je temu res tako. Z rezultati naših raziskav smo ugotovili, da so snovi, predvsem vitamin C, uspešni zaviralci rasti rakavih celic. Poizkus smo delali z jagodnim ekstraktom v etanolu in PBS-u. Oba sta se izkazala za zelo uspešna pri zaščiti celic pred citotoksičnostjo ter pri zaviranju rasti rakavih celic debelega črevesja (CaCo-2).
Keywords: jagode, rak, CaCo-2, antioksidanti, vitamin C
Published: 02.04.2012; Views: 1736; Downloads: 134
.pdf Full text (1,06 MB)

13.
Omega-3 maščobne kisline - učinkovina funkcionalnih živil, ki rešuje življenja
Gorazd Pisnik, 2012, master's thesis

Abstract: Namen naloge je bil ugotoviti poučenost koronarnih bolnikov o omega-3 maščobnih kislinah; koliko jih uživajo in koliko pozornosti jim namenjajo njihovi zdravniki. Raziskave kažejo, da lahko pomagajo preprečiti nastanek in razvoj obolenj, povezanih s previsokim nivojem vnetnih mehanizmov v telesu, zaradi neugodnega razmerja med omega-6 in omega-3 maščobnimi kislinami. Najmočneje vplivajo na srčno-žilni sistem, zato smo s pomočjo ankete raziskali prehranjevanje ljudi, ki se zdravijo zaradi koronarne bolezni srca. Ugotovili smo, da koronarni bolniki posvečajo veliko pozornosti zdravemu prehranjevanju, vendar uživajo premalo živil z omega-3 maščobnimi kislinami. Bolniki se ne poslužujejo funkcionalne prehrane in prehranskih dopolnil z omega-3 maščobnimi kislinami ter ne uživajo Omacor zdravil. Zdravniki pri zdravljenju teh bolnikov ne namenjajo pozornosti omega-3 maščobnim kislinam.
Keywords: Funkcionalna prehrana, omega-3 in omega-6 maščobne kisline, koronarna bolezen srca
Published: 25.05.2012; Views: 2113; Downloads: 289
.pdf Full text (1,71 MB)

14.
PRIPRAVA PODATKOVNE BAZE PROBIOTIČNIH IN PROTEKTURNIH KULTUR
Vanja Žiher, 2012, master's thesis

Abstract: Probiotične bakterije se med seboj razlikujejo. Ene so uspešnejše pri borbi proti škodljivim bakterijam, druge pri spodbujanju delovanja prebavil, tretje zopet pri spodbujanju imunskega sistema. Vse boljše poznavanje mehanizmov njihovega delovanja in vse več kliničnih raziskav, opravljenih v zadnjih letih, nakazuje možnost uspešne uporabe probiotičnih bakterij tako v preventivi, kot tudi podpori pri zdravljenju različnih bolezni. V magistrski nalogi, smo uporabili anketni vprašalnik in na podlagi pridobljenih rezultatov, smo opisovali lastnosti in klinično uporabo določenih probiotičnih sevov. Zbrani podatki, bodo predstavljeni na spletni strani Fakultete za Kmetijstvo in biosistemske vede Maribor.
Keywords: probiotiki, imunski sistem, Lactobacillus, Bifidobacterium, Enterococcus
Published: 25.05.2012; Views: 1520; Downloads: 212
.pdf Full text (1,97 MB)

15.
Hygiene monitoring of textiles used in the food industry
Sabina Fijan, Avrelija Cencič, Sonja Šostar-Turk, 2006, original scientific article

Abstract: Protective clothing is required in the food-processing industry, to protect workers from contamination by bacteria, fungi, viruses, prions etc. contained in the secretions and raw meat of slaughtered animals, and to protect the meat from being contaminated by microorganisms carried by the workers. It is well-understood that textiles are a control point (CP), and must be appropriately cleaned and disinfected in order to prevent biocontamination. Although the laundering procedure itself is important for achieving disinfection, it is also essential to maintain an appropriate hygiene level in the laundry, in order to prevent recontamination of textiles by environmental viable microorganisms. In this study, a sanitary-microbiologicalanalysis was carried out in selected CPs in two laundries. Chemo-thermal washing efficiency was determined by evaluating the anti-bacterial effect against Enterococcus faecium and Staphylococcus aureus. The hygienic state of the laundries was determined by evaluating the number and type of microorganisms at selected CPs throughout the whole laundering procedure. The results indicated that the sanitary condition of both laundries did not reach the required levels and that several microbes were resistant to cleaning and disinfecting agents. It is obvious from the results that achievement of an appropriate hygiene level during laundering textiles from the food processing industry requires the implementation of appropriate corrective monitoring measures.
Keywords: laundry hygiene, textiles, food-processing industry, occupational health
Published: 30.05.2012; Views: 1381; Downloads: 200
.pdf Full text (43,87 KB)
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Implementing hygiene monitoring systems in hospital laundries in order to reduce microbial contamination of hospital textiles
Sabina Fijan, Sonja Šostar-Turk, Avrelija Cencič, 2005, original scientific article

Abstract: As textiles sent to hospital laundries contain many types of pathogenic organisms, it is important that laundering not only has an appropriate cleaning effect but also has a satisfactory disinfecting effect. Critical to this process is the maintenance of an appropriate hygiene level in the clean area of laundries in order to prevent recontamination of textiles from manual handling when ironing, folding, packing etc. The aims of this study were to evaluate the hygienic state of a hospital laundry, to introduce continuous sanitary measures, and to introduce a continuous hygiene monitoring system with an infection control programme. Two systems for evaluating hospital laundry hygiene were combined: HACCP principles (hazard analysis and critical control points) and RAL-GZ 992 standards (quality assurance standard for textile care of hospital laundry). Evaluation of the hygienic state of the hospital laundry was carried out by evaluating the number and types of micro-organisms present at the critical control points throughout the whole laundering process, using RODAC agar plates for surface sampling and the pour plate method for investigating water samples. The initial examination showed that the sanitary condition of the laundry did not reach the required hygiene level. Therefore, fundamental sanitation measures were instituted and the examination was repeated. Results were then satisfactory. The most important critical control point was the chemothermal laundering efficiency of the laundering process. To prevent micro-organisms spreading into the entire cleanworking area, it is important that, in addition to regular sanitary measures such as cleaning/disinfecting all working areas, technical equipment and storage shelves etc., regular education sessions for laundry employees on proper hand hygiene is undertaken and effective separation of the clean and dirty working areas is achieved.
Keywords: textile care, hospital laundry, laundry hygiene, micro-organisms, washing quality parameters, sanitary microbiological parameters, hospital-acquired disease prevention, sanitary measures
Published: 01.06.2012; Views: 2028; Downloads: 52
URL Link to full text

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Rotaviral RNA found on various surfaces in a hospital laundry
Sabina Fijan, Andrej Steyer, Mateja Poljšak-Prijatelj, Avrelija Cencič, Sonja Šostar-Turk, Srečko Koren, 2008, original scientific article

Abstract: The aim of this investigative study was to determine the presence of rotaviral RNA at various control points (CP) of a hospital laundry. One of the possible sources of hospital infections is inappropriately laundered and disinfected hospital textiles. RT-PCR and nested PCR for gene amplification using specific primers following RNA isolation were used to determine the presence of rotaviral RNA on swabs. In addition, rotavirus suspensions were inoculated on marked surfaces as positive controls for different surfaces (cotton textiles, folding table and industrial dryer). Rotaviral RNA was found on various laundry surfaces: technical equipment, storage shelves, transport vehicles, personnel's hands, damp textiles, and folded laundry. Rotaviral RNA was also detected at all positive controls on tested surfaces after 24 h. Based on the results, it is very important to take into consideration the proper handling of textiles after washing as one of the precautions against hospital-acquired infections. This paper reports the presence of rotaviral RNA for the first time on surfaces in laundries and equipment, as well as textiles.
Keywords: laundry hygiene, rotaviruses, hospital-acquired disease prevention, sanitary measures, occupational health
Published: 01.06.2012; Views: 1802; Downloads: 57
URL Link to full text

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