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1.
Exploring chitosan-plant extract bilayer coatings: Advancements in active food packaging via polypropylene modification
Saša Kaloper, Olivija Plohl, Sonja Smole Možina, Alenka Vesel, Vida Šimat, Lidija Fras Zemljič, 2024, original scientific article

Abstract: UV-ozone activated polypropylene (PP) food films were subjected to a novel bilayer coating process involving primary or quaternary chitosan (CH/QCH) as the first layer and natural extracts from juniper needles (Juniperus oxycedrus; JUN) or blackberry leaves (Rubus fruticosus; BBL) as the second layer. This innovative approach aims to redefine active packaging (AP) development. Through a detailed analysis by surface characterization and bioactivity assessments (i.e., antioxidant and antimicrobial functionalities), we evaluated different coating combinations. Furthermore, we investigated the stability and barrier characteristics inherent in these coatings. The confirmed deposition, coupled with a comprehensive characterization of their composition and morphology, underscored the efficacy of the coatings. Our investigation included wettability assessment via contact angle (CA) measurements, X-ray photoelectron spectroscopy (XPS), and attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR), which revealed substantial enhancements in surface concentrations of elements and functional groups of CH, QCH, JUN, and BBL. Scanning electron microscopy (SEM) unveiled the coatings' heterogeneity, while time-of-flight secondary ion mass spectrometry (ToF-SIMS) and CA profiling showed moderately compact bilayers on PP, providing active species on the hydrophilic surface, respectively. The coatings significantly reduced the oxygen permeability. Additionally, single-layer depositions of CH and QCH remained below the overall migration limit (OML). Remarkably, the coatings exhibited robust antioxidative properties due to plant extracts and exceptional antimicrobial activity against S. aureus, attributed to QCH. These findings underscore the pivotal role of film surface properties in governing bioactive characteristics and offer a promising pathway for enhancing food packaging functionality.
Keywords: chitosan, plant extracts, polypropylene, active packaging, surface characterization, bioactivity
Published in DKUM: 27.05.2024; Views: 4; Downloads: 0
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2.
Entrepreneurship for Sustainable Future : GEM Slovenia 2023, Executive Summary
Karin Širec, Polona Tominc, Katja Crnogaj, Matej Rus, Barbara Bradač Hojnik, 2024, higher education textbook

Abstract: The GEM survey offers an in-depth analysis of the various social, cultural, political, and economic factors that impact the entrepreneurial landscape and activities, serving as a comprehensive framework for understanding national and entrepreneurial conditions. These factors play a crucial role in shaping individuals' entrepreneurial decisions and the success of starting and operating businesses. Survey delivers unparalleled insights into a nation's entrepreneurial characteristics, surpassing the information available from traditional statistical sources. The study also explores individuals' and societies' attitudes towards entrepreneurship, alongside the aspirations and qualifications of people at different entrepreneurial stages. By examining these aspects across countries, GEM identifies key drivers and obstacles for entrepreneurship, enabling stakeholders to cultivate a supportive entrepreneurial ecosystem. The monograph also addresses the growing link between entrepreneurial endeavors and sustainable development. The research emphasizes the importance of sustainable development as an integral part of entrepreneurial thinking and action in Slovenia, which is key to promoting innovation, competitiveness and long-term prosperity in
Keywords: Global Entrepreneurship Monitor, entrepreneurship, early-stage entrepreneurial activity, economic development, entrepreneurship ecosystem, entrepreneurship policy, sustainable development
Published in DKUM: 14.05.2024; Views: 64; Downloads: 5
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3.
Antimicrobial properties of different hop (Humulus lupulus) genotypes
Zala Kolenc, Tomaž Langerholc, Gregor Hostnik, Miha Ocvirk, Sara Štumpf, Maša Pintarič, Iztok Jože Košir, Andreja Čerenak, Alenka Garmut, Urban Bren, 2023, original scientific article

Abstract: The antimicrobial activity of hop extracts obtained from different hop genotypes were investigated against Staphylococcus aureus and Lactobacillus acidophilus. In this study the pure xanthohumol, purified β-acids rich fraction, as well as α-acids with β-acids rich fraction were used to test antimicrobial activity against Staphylococcus aureus and Lactobacillus acidophilus; whereby, the antimicrobial activity of different hop extracts against Lactobacillus acidophilus was studied for the first time. Microbial susceptibility to purified hydroacetonic extracts from different hop varieties was investigated by the broth microdilution assay to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The hop hydroacetonic extracts were more effective against Staphylococcus aureus than against Lactobacillus acidophilus. Strong inverse correlations of MIC and MBC values were obtained with xanthohumol, cohumulone, n+adhumulone, colupulone and n+adlupulone contents, suggesting that the identified chemical hop compounds are directly responsible for antimicrobial effects. Moreover, the effect of the growth medium strength on the MIC values of hop extracts against Staphylococcus aureus was systematically investigated for the first time. The current study also reveals the effect of different hop extracts on Staphylococcus aureus, which responds to their presence by lag phase extension and generation time prolongation.
Keywords: hop extract, xanthohumol, Staphylococcus aureus, Lactobacillus acidophilus, minimum inhibitory concentration, minimum bactericidal concentration
Published in DKUM: 09.05.2024; Views: 91; Downloads: 3
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4.
Podjetništvo za trajnostno prihodnost : GEM Slovenija 2023
Karin Širec, Polona Tominc, Katja Crnogaj, Matej Rus, Barbara Bradač Hojnik, 2024, higher education textbook

Abstract: Raziskava GEM ponuja poglobljeno analizo različnih družbenih, kulturnih, političnih in ekonomskih dejavnikov, ki vplivajo na podjetniško okolje in podjetniško aktivnost, ter predstavlja celovit okvir za razumevanje nacionalnih podjetniških razmer. Ti dejavniki imajo ključno vlogo pri oblikovanju podjetniških ambicij posameznikov in uspešnosti ustanavljanja ter delovanja podjetij. Raziskava s tem zagotavlja neprimerljiv vpogled v nacionalno podjetništvo in njegove značilnosti ter presega informacije, ki so na voljo v drugih statističnih virih. Raziskava namreč vključuje tudi odnos posameznikov in družbe do podjetništva ter želje in usposobljenost ljudi za podjetništvo, v vseh fazah podjetniškega procesa. S proučevanjem teh vidikov v različnih državah, GEM ugotavlja ključna gonila in ovire za podjetništvo, kar zainteresiranim stranem omogoča, da razvijajo podporni podjetniški ekosistem. Monografija se dotika tudi vse večje povezave med podjetniškimi prizadevanji in trajnostnim razvojem. V raziskavi je poudarjen pomen trajnostnega razvoja kot integralnega dela podjetniške miselnosti in prakse v Sloveniji, kar je ključno za spodbujanje inovativnosti, konkurenčnosti in dolgoročne blaginje v družbi.
Keywords: Globalni podjetniški monitor, podjetništvo, celotna zgodnja podjetniška aktivnost, gospodarski razvoj, podjetniški ekosistem, podjetniška politika, trajnostni razvoj
Published in DKUM: 06.05.2024; Views: 66; Downloads: 9
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5.
Podjetniška demografija in značilnosti podjetništva mladih : Slovenski podjetniški observatorij 2023
Karin Širec, Barbara Bradač Hojnik, Dijana Močnik, 2024

Abstract: V okviru Slovenskega podjetniškega observatorija 2023 smo proučevali demografijo slovenskih podjetij in značilnosti podjetništva mladih v Sloveniji in primerjalno z Evropo. Kot vsako leto, smo tudi tokrat v prvem delu raziskave Slovenskega podjetniškega observatorija analizirali demografske značilnosti slovenskih podjetij, medtem ko smo drugi del posvetili aktualni temi tako v slovenskem kot evropskem gospodarstvu, analizi podjetništva mladih. Demografska analiza je bila usmerjena v razumevanje profilov slovenskih podjetij na podlagi različnih kriterijev, kot so število podjetij, njihova velikost, ustvarjen prihodek, stroški dela in dodana vrednost, razčlenjeno po dejavnosti, regiji in velikosti podjetja. Proučevanje značilnosti podjetništva mladih je zagotovilo vpogled v dinamiko podjetništva v izbrani ciljni skupini z namenom oblikovanja učinkovitih ukrepov spodbujanja razvoja podjetništva mladih.
Keywords: gospodarske družbe, samostojni podjetniki, kazalniki finančne uspešnosti, podjetništvo mladih, značilnosti, priložnosti in ovire, strategije in politike, empirična analiza
Published in DKUM: 24.04.2024; Views: 93; Downloads: 9
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6.
Malignomu podoben vnetni pseudotumor: redek zaplet popolne artroplastike kolka
Alenka Repše-Fokter, Samo K. Fokter, 2009, original scientific article

Abstract: Purpose: Particulate debris created by accelerated polyethylene wear in totaly replaced hips causes adverse local reactions. We report an extreme form of such a reaction in a patient who developed progressive thigh pain, weakness and numbness after total arthroplasty of his left hip. Methods: An abdominopelvic computed tomogram showed a large intrepelvic cystic mass interpreted as a malignant tumor. Dark greyish-green fluid was aspirated from the cyst and cytopathologic examination showed necrotic debris without malignant cells. Biopsy revealed necrosis and abundant foreign body granulation tissue with polarizable debris. Results: During surgical removal of the cyst a defect of the inner acetabular wall was noted. After successful revision arthroplasty with allograft bone the clinical symptoms improved. Conclusion: A foreign-body reaction to wear debris may produce an intrapelvic mass and corresponding symptoms. Cytologic and histologic examination of the mass or fluid may aid in interpreting the clinical picture and imaging studies.
Keywords: totalna artroplastika kolka, nekrotični drobir, medenični tumor, reakcija tujkovega tipa
Published in DKUM: 19.04.2024; Views: 62; Downloads: 1
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7.
Detection of aneuploidy using multiplex ligation-dependent probe amplification in fetal tissues from aborted pregnancies
Boris Zagradišnik, Špela Stangler Herodež, Alenka Erjavec Škerget, Andreja Zagorac, Nadja Kokalj-Vokač, 2011, original scientific article

Abstract: Purpose: About 10-15% of all pregnancies terminate as spontaneous miscarriages. In the first trimester, 50% of spontaneous miscarriages are the result of chromosomal aberrations, mostly chromosomal aneuploidies. Cytogenetic analyses are used to confirm aneuploidy in failed pregnancies. Culture failure or poor quality chromosomes are often problems in those cases. In such situations, methods that are independent of tissue culture areused, and we employed multiplex ligation-dependent probe amplification (MLPA). We determined if MLPA is an appropriate and compatible method compared with classical cytogenetic analyses on fetal tissues. Methods: All fetal samples received from spontaneous abortions were cultured, karyotyped (if possible) and genomic DNA extracted. MLPA analyses were undertaken using subtelomeric probe kits. Additionally, comparative genomic hybridization (CGH) was used to confirm aneuploidy detected by MLPA in cases of failed culture growth. Results: MLPA analyses confirmed an unbalanced chromosome abnormality identified by cytogenetic analyses in all cases in which tissue culture was successful, and provided data in cases of failed culture growth. Several common numeric chromosome aberrations were detected, as well as rare trisomies and other unbalanced chromosome rearrangements. Conclusions: MLPA analyses can provide information about the karyotype of a DNA sample if cytogenetic analyses are not possible because of a lack of viable cells or if only a small amount of genomic DNA is available. These data indicate that MLPA may also be a very useful method for early prenatal aneuploidy screening.
Keywords: pomnoževanje od ligacije odvisnih prob, številčne kromosomske preureditve, kariotip
Published in DKUM: 12.04.2024; Views: 112; Downloads: 2
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8.
Detection of vkorc1 polymorphism : comparison of polymerase chain reaction/restriction fragment length polymorphism (pcr + rflp) with allele-specific polymerase chain reaction
Špela Stangler Herodež, Nastja Stankovič, Boris Zagradišnik, Alenka Erjavec Škerget, Nadja Kokalj-Vokač, 2013, original scientific article

Abstract: Purpose: The VKORC1 polymorphism is an important genetic factor affecting warfarin dose requirement. Patients require different warfarin doses in order to achieve the target therapeutic anticoagulation. The aim of our study was to determine the frequency of single nucleotide polymorphisms (SNP) in the VKORC1gene in the general population, using a simple, rapid, and economical method. Methods: For genotyping, the restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR) amplified DNA was used and compared to allele specific polymerase chain reaction. We genotyped 441 DNA samples obtained from the healthy general population in North Eastern Slovenia. Genotypes for the tested group were evaluated to determine whether the population followed the Hardy Weinberg equilibrium. The genotypes and allele frequencies were calculated. Results: The results obtained using the allele specific polymerase chain reaction were consistent with those obtained using the PCR + RFLP method. The G allele frequency (0.62) was higher than the A allele frequency (0.38) in the general population from North Eastern Slovenia. Conclusions: The PCR+RFLP method involved additional manipulation of the PCR products at the expense of analysis time, consumption of reagents and equipment. The allele specific polymerase chain reaction was a simple and rapid method for the detection of SNP in theVKORC1 gene, and is available in any laboratory with the minimum of equipment and reagents required.
Keywords: VKORC1, varfarin, PCR, RFLP, alelno specifična verižna reakcija s polimerazo
Published in DKUM: 12.04.2024; Views: 69; Downloads: 2
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9.
Funkcionalna specifikacija aplikacije za preprečevanje pojava karpalnega sindroma
Blaž Žirovnik, 2024, undergraduate thesis

Abstract: V diplomski nalogi smo pripravili funkcionalne specifikacije za aplikacijo, namenjeno preprečevanju karpalnega sindroma. Po pregledu obstoječih rešitev na trgu, ki večinoma niso ustrezale zaradi zastarelosti, omejenega nabora vaj ali omejenega dostopa, smo se odločili pripraviti specifikacije za novo rešitev. Cilj diplomskega dela je bil oblikovati funkcionalne specifikacije za izdelavo aplikacije, ki bo pomagala preprečevati pojav karpalnega sindroma. V skladu s ciljem diplomskega dela smo tako najprej predstavili mobilne aplikacije in mobilne operacijske sisteme ter orodja za razvoj aplikacij. Posebno pozornost smo namenili mobilnim aplikacijam s področja zdravstva. Na osnovi pregleda obstoječih aplikacij, njihovih prednosti in pomanjkljivosti, smo pripravili prototip aplikacije, ki ponuja vaje za preprečevanje karpalnega sindroma. Aplikacija bo vključevala podrobnejši prikaz vaj, nastavitev opomnikov za izvajanje vaj ter pošiljanje povratnih informacij o uporabnosti aplikacije.
Keywords: mobilna aplikacija, funkcionalne specifikacije, karpalni sindrom
Published in DKUM: 09.04.2024; Views: 96; Downloads: 7
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10.
Correlations between infant mortality and recommended immunization schedules against vaccine-preventable diseases in Slovenia
Mateja Rakuša, Božena Pejković, Ivan Krajnc, Alenka Kraigher, 2017, original scientific article

Keywords: program cepljenja, nalezljive bolezni, vzroki smrti, stopnja umrljivosti dojenčkov
Published in DKUM: 09.04.2024; Views: 74; Downloads: 4
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