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Naslov:Microbiota in vitro modulated with polyphenols shows decreased colonization resistance against Clostridioides difficile but can neutralize cytotoxicity
Avtorji:ID Mahnič, Aleksander (Avtor)
ID Auchtung, Jennifer (Avtor)
ID Poklar Ulrih, Nataša (Avtor)
ID Britton, Robert A. (Avtor)
ID Rupnik, Maja (Avtor)
Datoteke:.pdf Mahnic-2020-Microbiota__i_in_vitro__i__modulat.pdf (1,67 MB)
MD5: 2CC00F4AB632A741987E0AB2C703F5C0
 
URL https://doi.org/10.1038/s41598-020-65253-0
 
Jezik:Angleški jezik
Vrsta gradiva:Znanstveno delo
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:MF - Medicinska fakulteta
Opis:While the knowledge on gut microbiota - C. difficile interactions has improved over the years, the understanding of the underlying mechanisms providing colonization resistance as well as preventative measures against the infection remain incomplete. In this study the antibiotic clindamycin and polyphenol extracts from pomegranate and blueberries were used individually and in combination to modulate fecal microbial communities in minibioreactor arrays (MBRA). Modulated communities were inoculated with C. difficile (ribotype 027). Subsequent 7-day periodical monitoring included evaluation of C. difficile growth and activity of toxins TcdA and TcdB as well as analysis of MBRA bacterial community structure (V3V4 16%S metagenomics). Polyphenols affected multiple commensal bacterial groups and showed different synergistic and antagonistic effects in combination with clindamycin. Exposure to either clindamycin or polyphenols led to the loss of colonization resistance against C. difficile. The successful growth of C. difficile was most significantly correlated with the decrease in Collinsella and Lachnospiraceae. Additionally, we demonstrated that Clostridium sporogenes decreased the activity of both C. difficile toxins TcdA and TcdB. The feature was shown to be common among distinct C. sporogenes strains and could potentially be applicable as a non-antibiotic agent for the alleviation of C. difficile infection.
Ključne besede:microbiota in vitro, Clostridioides difficile, resistance, cytotoxicity
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Poslano v recenzijo:13.01.2020
Datum sprejetja članka:21.03.2020
Datum objave:20.05.2020
Založnik:Nature Publishing Group
Leto izida:2020
Št. strani:Str. 1-11
Številčenje:Letn. 10, št. 8358
PID:20.500.12556/DKUM-91707 Novo okno
UDK:579.852.13
COBISS.SI-ID:16205827 Novo okno
DOI:10.1038/s41598-020-65253-0 Novo okno
ISSN pri članku:2045-2322
Datum objave v DKUM:29.01.2025
Število ogledov:0
Število prenosov:3
Metapodatki:XML DC-XML DC-RDF
Področja:Ostalo
:
MAHNIČ, Aleksander, AUCHTUNG, Jennifer, POKLAR ULRIH, Nataša, BRITTON, Robert A. in RUPNIK, Maja, 2020, Microbiota in vitro modulated with polyphenols shows decreased colonization resistance against Clostridioides difficile but can neutralize cytotoxicity. Scientific reports [na spletu]. 2020. Vol. 10, no. 8358, p. 1–11. [Dostopano 4 april 2025]. DOI 10.1038/s41598-020-65253-0. Pridobljeno s: https://dk.um.si/IzpisGradiva.php?lang=slv&id=91707
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Gradivo je del revije

Naslov:Scientific reports
Skrajšan naslov:Sci. rep.
Založnik:Nature Publishing Group
ISSN:2045-2322
COBISS.SI-ID:18727432 Novo okno

Gradivo je financirano iz projekta

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:P3-0387
Naslov:Črevesna mikrobiota - vloga v zdravju in pri boleznih
Financer:Drugi - Drug financer ali več financerjev
Številka projekta:FEMS-RG-2016-0048

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:20.05.2020

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:mikrobiota, C. difficile, odpornost, citotoksičnost


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