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Naslov:Platelet-rich plasma, especially when combined with a TGF-ß inhibitor promotes proliferation, viability and myogenic differentiation of myoblasts in vitro
Avtorji:ID Kelc, Robi (Avtor)
ID Trapečar, Martin (Avtor)
ID Gradišnik, Lidija (Avtor)
ID Rupnik, Marjan (Avtor)
ID Vogrin, Matjaž (Avtor)
Datoteke:.pdf PLOS_ONE_2015_Kelc_et_al._Platelet-Rich_Plasma,_Especially_When_Combined_with_a_TGF-β_Inhibitor_Promotes_Proliferation,_Viability_and_My.PDF (2,07 MB)
MD5: 803D5040EBB2FC648FF63974EC178847
 
URL http://dx.plos.org/10.1371/journal.pone.0117302
 
Jezik:Angleški jezik
Vrsta gradiva:Znanstveno delo
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:MF - Medicinska fakulteta
Opis:Regeneration of skeletal muscle after injury is limited by scar formation, slow healing time and a high recurrence rate. A therapy based on platelet-rich plasma (PRP) has become a promising lead for tendon and ligament injuries in recent years, however concerns have been raised that PRP-derived TGF-β could contribute to fibrotic remodelling in skeletal muscle after injury. Due to the lack of scientific grounds for a PRP -based muscle regeneration therapy, we have designed a study using human myogenic progenitors and evaluated the potential of PRP alone and in combination with decorin (a TGF-β inhibitor), to alter myoblast proliferation, metabolic activity, cytokine profile and expression of myogenic regulatory factors (MRFs). Advanced imaging multicolor single-cell analysis enabled us to create a valuable picture on the ratio of quiescent, activated and terminally committed myoblasts in treated versus control cell populations. Finally high-resolution confocal microscopy validated the potential of PRP and decorin to stimulate the formation of polynucleated myotubules. PRP was shown to down-regulate fibrotic cytokines, increase cell viability and proliferation, enhance the expression of MRFs, and contribute to a significant myogenic shift during differentiation. When combined with decorin further synergistc effects were identified. These results suggest that PRP could not only prevent fibrosis but could also stimulate muscle commitment, especially when combined with a TGF-β inhibitor.
Ključne besede:muscles, skeletal, injuries, TGF-beta, plasma, thrombocytes, myoblasts, fibrosis, prevention, regeneration
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Leto izida:2015
Št. strani:str. 1-13
Številčenje:Letn. 10, št. 2
PID:20.500.12556/DKUM-66288 Novo okno
ISSN:1932-6203
UDK:616-001
COBISS.SI-ID:5269311 Novo okno
DOI:10.1371/journal.pone.0117302 Novo okno
ISSN pri članku:1932-6203
NUK URN:URN:SI:UM:DK:J1A0HJD7
Datum objave v DKUM:19.06.2017
Število ogledov:1459
Število prenosov:187
Metapodatki:XML DC-XML DC-RDF
Področja:Ostalo
:
KELC, Robi, TRAPEČAR, Martin, GRADIŠNIK, Lidija, RUPNIK, Marjan in VOGRIN, Matjaž, 2015, Platelet-rich plasma, especially when combined with a TGF-ß inhibitor promotes proliferation, viability and myogenic differentiation of myoblasts in vitro. PloS one [na spletu]. 2015. Vol. 10, no. 2, p. 1–13. [Dostopano 4 april 2025]. DOI 10.1371/journal.pone.0117302. Pridobljeno s: https://dk.um.si/IzpisGradiva.php?lang=slv&id=66288
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Gradivo je del revije

Naslov:PloS one
Založnik:Public Library of Science
ISSN:1932-6203
COBISS.SI-ID:2005896 Novo okno

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:19.06.2017

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:mišice, skeletne, poškodbe, TGF-beta, plazma, bogata s trombociti, mioblasti, fibroza, preprečevanje, regeneracija


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